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Human MICA Antibody

Catalog #: MAB13002
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB13002-SP
MAB13002-500
MAB13002-100
Product Details
Citations (2)
FAQs
Supplemental Products
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MICA Antibody Summary

Species Reactivity
Human
Specificity
Detects human MICA in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human MICB is observed.
Source
Monoclonal Mouse IgG1 Clone # 159233
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human MICA
Accession # Q29983
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MICA Fc Chimera (Catalog # 1300-MA)
under non-reducing conditions only

Human MICA Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human MICA Biotinylated Antibody (Catalog # BAF1300)

Standard: Recombinant Human MICA Fc Chimera Protein, CF (Catalog # 1300-MA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MICA

MHC class I Chain-related gene A (MICA) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

Long Name
MHC Class I-related Protein A
Entrez Gene IDs
100507436 (Human)
Alternate Names
FLJ60820; MGC111087; MICA; PERB11.1

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Citations for Human MICA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Selective induction of host genes by MVA-B, a candidate vaccine against HIV/AIDS.
    Authors: Guerra S, Gonzalez JM, Climent N, Reyburn H, Lopez-Fernandez LA, Najera JL, Gomez CE, Garcia F, Gatell JM, Gallart T, Esteban M
    J. Virol., 2010-06-09;84(16):8141-52.
    Species: Mouse
    Sample Types: Cell Culture Supernates, Cell Lysates, Whole Cells
    Applications: ELISA Development, ICC, Western Blot
  2. A simple immunoassay for extracellular vesicle liquid biopsy in microliters of non-processed plasma
    Authors: Carmen Campos-Silva, Yaiza Cáceres-Martell, Estela Sánchez-Herrero, Amaia Sandúa, Alexandra Beneitez-Martínez, Álvaro González et al.
    Journal of Nanobiotechnology

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