Human MICL/CLEC12A Antibody

Catalog #: AF2946 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2946
AF2946-SP

All MICL/CLEC12A Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MICL/CLEC12A Antibody Summary

Species Reactivity

Human

Specificity

Detects human MICL/CLEC12A in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse MICL is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MICL/CLEC12A isoform a
Thr67-Ala265
Accession # NP_612210

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human MICL/CLEC12A

Immunocytochemistry

5-15 µg/mL

Immersion fixed activated human peripheral blood mononuclear cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MICL/CLEC12A

MICL, also known as C-type lectin-like molecule-1 (CLL-1), Killer cell lectin-like receptor KLRL1 and C type lectin domain family 12, member A (CLEC12A), is a type II transmembrane highly glycosylated protein that contains a functional tyrosine-based inhibitory motif in its cytoplasmic tail. The gene for MICL has been mapped to the natural killer gene complex (NKC) on human chromosome 12p13.2. MICL contains a non-classic C-type domain that binds non-sugar ligands. It is primarily expressed on NK cells, T cells, dendritic cells, monocytes and macrophages. By alternative splicing, multiple splice isoforms exist. MICL is a negative regulator of granulocytes and monocyte function and inhibits NK cell cytotoxicity. The amino acid sequence of human MICL extracellular domain is 53%, 65% and 55% identical to that of mouse, canine and bovine MICL, respectively.

Long Name

Myeloid Inhibitory C-type Lectin-like Receptor

Entrez Gene IDs

160364 (Human); 232413 (Mouse)

Alternate Names

CD371; CLEC12A; CLL1; CLL-1; C-type lectin domain family 12, member A; DCAL-2; MICL