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Human MMP-8 Magnetic Luminex® Performance Assay

Catalog #: LMPM908
3 Citations Datasheet / COA / SDS

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Quantist software graph of signal intensity versus concentration for MMP-8 in the Human MMP Luminex Panel
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Summary Configure Your Assay Product Features Precision & Accuracy Data Examples Product Datasheets Background

Human MMP-8 Magnetic Luminex® Performance Assay Summary

Panel Luminex Performance Human MMP Panel
Assay Type Magnetic bead-based multiplex assay for the Luminex® platform
Format 1 x 96-well microplate and Magnetic antibody-coated beads
Species Human
Analytes Detected Please see analyte list in assay customization tool below.
Performance Validation Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

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Assays

Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If you run into bead region incompatibilities, send your Luminex code to our team and find out more.

How would you like these analytes mixed?

Premixed
User Mixed
Please select at least one mixing option to see the kits.

Would you like to split based on dilution?

Do not split
Split based on serum
Split based on plasma
Split based on cell culture supernates

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.

Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

  • Simultaneously profile Human MMP-8 with up to analytes of your choice in one sample
  • Magnetic format allows for easier wash steps
  • Undergoes similar validation testing as Quantikine® ELISA Kits
  • Precise and Specific
  • Requires a small sample volume (<50 µL)
  • Run your assay in just 3-5 hours
  • Mass-calibrated standards for consistent results with every new lot of material
  • Enough reagents for one 96-well plate
Base Kit Components
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Calibrator Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One flat-bottom 96-well Microplate
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Standard Value Card**

**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.

Bead Set Contents
  • User-mix antibody-coated Magnetic beads in individual vials
  • User-mixed cocktail of biotinylated detection antibody

Precision

Intra-assay Precision (precision within an assay) - Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (precision between assays) - Three samples of known concentration were tested in fifty-nine separate assays to assess precision between assays.
  Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 59 59 59
Mean (pg/mL) 558 5177 23,073 565 4888 20,584
Standard deviation 39.2 271 1410 81.0 481 1977
CV (%) 7.0 5.2 6.1 14.3 9.8 9.6
 

Recovery

Samples containing and/or spiked with human MMP-8 were evaluated for recovery.
Sample Type Average % Recovery Range (%)
Cell culture supernate 109 72-134
Serum 85 72-95
Heparin plasma 86 67-97
Platelet-poor heparin plasma 85 64-111
Urine 110 88-131
 

Linearity

Samples containing and/or spiked with human MMP-8 were serially diluted to evaluate assay linearity.
  Cell culture supernate Serum Heparin plasma Platelet-poor heparin plasma Saliva Urine
1:2 Average % of Expected 104 112 113 111 107 104
Range (%) 95-112 107-118 105-119 100-128 92-117 90-117
1:4 Average % of Expected 94 110 114 103 96 91
Range (%) 85-102 103-127 104-123 91-113 83-104 75-98
1:8 Average % of Expected 83 109 107 96 99 85
Range (%) 74-93 99-127 98-123 84-113 85-109 71-93
Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.

Scientific Data

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Quantist software graph of signal intensity versus concentration for MMP-8 in the Human MMP Luminex Panel

Background: MMP-8

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name
Matrix Metalloproteinase 8
Entrez Gene IDs
4317 (Human); 17394 (Mouse); 63849 (Rat)
Alternate Names
CLG1HNC; Collagenase 2; EC 3.4.24; EC 3.4.24.34; matrix metallopeptidase 8 (neutrophil collagenase); matrix metalloproteinase 8 (neutrophil collagenase); Matrix metalloproteinase-8; MMP8; MMP-8; neutrophil collagenase; PMNL collagenase; PMNL-CL

Product Datasheets

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Product Specific Notices
Luminex® is a registered trademark of Luminex Corporation.

Citations for Human MMP-8 Magnetic Luminex® Performance Assay

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Plasma levels of C-reactive protein, matrix metalloproteinase-7 and lipopolysaccharide-binding protein distinguish active pulmonary or extrapulmonary tuberculosis from uninfected controls in children
    Authors: VVS Albuquerqu, NP Kumar, KF Fukutani, B Vasconcelo, MB Arriaga, PS Silveira-M, S Babu, BB Andrade
    Cytokine, 2019-07-09;123(0):154773.
    Species: Human
    Sample Types: Plasma
  2. Tumor Necrosis Factor-alpha, Matrix-Metalloproteinases 8 and 9 Levels in the Saliva Are Associated with Increased Hemoglobin A1c in Type 1 Diabetes Subjects.
    Authors: Kuehl M, Rodriguez H, Burkhardt B, Alman A
    PLoS ONE, 2015-04-27;10(4):e0125320.
    Species: Human
    Sample Types: Saliva
  3. Gender-dependent differences in plasma matrix metalloproteinase-8 elevated in pulmonary tuberculosis.
    Authors: Sathyamoorthy T, Sandhu G, Tezera L, Thomas R, Singhania A, Woelk C, Dimitrov B, Agranoff D, Evans C, Friedland J, Elkington P
    PLoS ONE, 2015-01-30;10(1):e0117605.
    Species: Human
    Sample Types: Plasma

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