Human MMP-9/TIMP-1 Complex DuoSet ELISA

Name: Human MMP-9/TIMP-1 Complex DuoSet ELISA
Brand: R&D Systems
SKU: DY1449
Rating: 5 (1 reviews)

Catalog #: DY1449
8 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
DY1449

Ancillary Products Available

DY999B: Substrate Reagent Pack

DY999: Substrate Reagent Pack

Human MMP-9 / TIMP-1 Complex ELISA Standard Curve

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All MMP-9/TIMP-1 Complex ELISAs

Product Details

Citations (8)

FAQs

Supplemental Products

Reviews (1)

Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage

Human MMP-9/TIMP-1 Complex DuoSet ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Sample Volume Required

100 µL

Assay Range

46.9 - 3,000 pg/mL

Sufficient Materials

For fifteen 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human MMP-9 / TIMP-1 Complex. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A

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Human MMP-9 / TIMP-1 Complex ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Citations for Human MMP-9/TIMP-1 Complex DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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The Biologic IRL201805 Alters Immune Tolerance Leading to Prolonged Pharmacodynamics and Efficacy in Rheumatoid Arthritis Patients
Authors: Hall, C;Pleasance, J;Hickman, O;Kirkham, B;Panayi, GS;Eggleton, P;Corrigall, VM;
International journal of molecular sciences
Species: Human
Sample Types: Cell Culture Supernates
Implications of the Matrix Metalloproteinases, Their Tissue Inhibitors and Some Other Inflammatory Mediators Expression Levels in Children Obesity-Related Phenotypes
Authors: Wierzbicka-Ruci?ska, A;Kubiszewska, I;Grzywa-Czuba, R;Gackowska, L;Szalecki, M;Micha?kiewicz, J;Trojanek, JB;
Journal of personalized medicine
Species: Human
Sample Types: Cell Culture Supernates, Plasma
The diagnostic and prognostic potential of the EGFR/MUC4/MMP9 axis in glioma patients
Authors: A Quesnel, N Coles, TM Polvikoski, GS Karagianni, C Angione, M Islam, AA Khundakar, PS Filippou
Scientific Reports, 2022-11-18;12(1):19868.
Species: Human
Sample Types: Serum
Atypical response to bacterial co-infection and persistent neutrophilic broncho-alveolar inflammation distinguish critical COVID-19 from influenza
Authors: S Cambier, M Metzemaeke, AC Carvalho, A Nooyens, C Jacobs, L Vanderbeke, B Malengier-, M Gouwy, E Heylen, P Meersseman, G Hermans, E Wauters, A Wilmer, C Consortium, D Schols, P Matthys, G Opdenakker, RE Marques, J Wauters, J Vandooren, P Proost
JCI Insight, 2022-01-11;0(0):.
Species: Human
Sample Types: BAL Supernatant
MMP-9 Levels and IMT of Carotid Arteries are Elevated in Obese Children and Adolescents Compared to Non-Obese
Authors: C Andrade, A Bosco, V Sandrim, F Silva
Arq. Bras. Cardiol., 2017-03-01;108(3):198-203.
Species: Human
Sample Types: Plasma
Evaluation of Association Between the Serum Levels of MMP-9 and MMP-9/TIMPs With Soluble Forms of Selectins and Itching Induced by Sulfur Mustard
Authors: N Askari, T Ghazanfari, MM Naghizadeh, A Moin, A Khamesipou, S Pourfarzam, ZM Hassan
Iran J Pathol, 2017-01-27;12(3):257-264.
Species: Human
Sample Types: Serum
Plasma levels of the MMP-9:TIMP-1 complex as prognostic biomarker in breast cancer: a retrospective study.
Authors: Thorsen S, Christensen S, Wurtz S, Lundberg M, Nielsen B, Vinther L, Knowles M, Gee N, Fredriksson S, Moller S, Brunner N, Schrohl A, Stenvang J
BMC Cancer, 2013-12-13;13(0):598.
Species: Human
Sample Types: Plasma
Differential proteolytic enzyme activity in eosinophilic and neutrophilic asthma.
Authors: Simpson JL, Scott RJ, Boyle MJ, Gibson PG
Am. J. Respir. Crit. Care Med., 2005-05-18;172(5):559-65.
Species: Human
Sample Types: Sputum