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Human/Mouse ADAMTS1 Antibody

Catalog #: AF5867
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5867
AF5867-SP
Detection of Human and Mouse ADAMTS1 by Western Blot.
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Citations (10)
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Human/Mouse ADAMTS1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse ADAMTS1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) ADAMTS4 and rhADAMTS15 is observed and less than 1% cross‑reactivity with rhADAMTSL1.2, rhADAMTSL2, rhADAMTS5, rhADAMTS8, rhADAMTS10, rhADAMTS12, rhADAMTS13, and rhADAMTS16 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse ADAMTS1
Phe254-Cys725
Accession # P97857
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse ADAMTS1 (Catalog # 5867-AD), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse ADAMTS1 antibody by Western Blot. View Larger

Detection of Human and Mouse ADAMTS1 by Western Blot. Western blot shows lysates of P19 mouse embryonal carcinoma cell line and OVCAR-3 human ovarian carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse ADAMTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5867) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ADAMTS1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry ADAMTS1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

ADAMTS1 in Mouse Embryo. ADAMTS1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Human/Mouse ADAMTS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5867) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cartilage primordium; dorsal ganglia cell bodies and processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunocytochemistry/ Immunofluorescence Detection of Human ADAMTS1 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human ADAMTS1 by Immunocytochemistry/Immunofluorescence Immunohistochemical staining.(A) ADAMTS-1, (B) SMC marker alpha -actin, (C) macrophage marker in adventitial layer of human AAA aortas and (D) ADAMTS-1 in human non-aneurysmal control aortas. Staining of positive cells are seen in brown. The images are taken with 40X magnification and scale bar represent 50 μm. Red arrow indicates the same area. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28570682), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human ADAMTS1 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human ADAMTS1 by Immunocytochemistry/Immunofluorescence Immunohistochemical staining.(A) ADAMTS-1, (B) SMC marker alpha -actin, (C) macrophage marker in adventitial layer of human AAA aortas and (D) ADAMTS-1 in human non-aneurysmal control aortas. Staining of positive cells are seen in brown. The images are taken with 40X magnification and scale bar represent 50 μm. Red arrow indicates the same area. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28570682), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human ADAMTS1 by Western Blot View Larger

Detection of Human ADAMTS1 by Western Blot Western blot analysis.(A) Expression of ADAMTS-1 and GAPDH in human AAA aortas (sample 1–6) and human non-aneurysmal control aortas (sample 7–11) and (B) quantification of ADAMTS-1 in relative values normalized to GAPDH. MWM: molecular weight marker indicating size in kDa. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28570682), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAMTS1

ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1), also known as METH1, is the founding member of the family of secreted zinc proteases with a multidomain structure (1-3). The protein precursor consists of a signal peptide and the following domains: pro, catalytic, disintegrinlike, TS type 1 motif, cysteine rich, spacer and a variable number of thrombospondin type 1 motifs. Based on their substrate specificity, ADAMTS1 and associated family members may be key enzymes in the degradation of cartilage leading to inflammation and arthritis (4). It is an active protease cleaving alpha 2macroglobulin (5), aggrecan (6), and versican (7). Compared to ADAMTS4 (aggrecanase 1) and ADAMTS5 (aggrecanase 2), the aggrecanase activity of ADAMTS1 is lower. However, its activity can be enhanced by the binding of a cofactor such as fibulin1 (8). ADAMTS1 is essential for normal growth and the structure and function of the kidneys, adrenal glands and female reproductive organs (9). It also plays an important role in atherosclerosis (10). It has been shown to inhibit endothelial cell proliferation by direct binding and sequestration of VEGF165 and to inhibit fibroblast migration at high concentrations by binding to FGF 2 (11, 12). The purified rmADAMTS1 starts at the N terminus of the catalytic domain and ends in the Cys-rich domain.

References
  1. Vazquez, F. et al. (1999) J. Biol. Chem. 274:23349.
  2. Kuno, K. et al. (1997) J. Biol. Chem. 272:556.
  3. Porter, S. et al. (2005) Biochem. J. 386:15.
  4. Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
  5. Kuno, K. et al. (1999) J. Biol. Chem. 274:18821.
  6. Kuno, K. et al. (2000) FEBS Lett. 478:241.
  7. Russel, D. L. et al. (2003) J. Biol. Chem. 278:42330.
  8. Lee, N. et al. (2005) J. Biol. Chem. 280:34796.
  9. Shindo, T. et al. (2000) J. Clin. Invest. 105:1345.
  10. Wight, T.N. (2005) Arterioscler Thromb. Vasc. Biol. 25:12.
  11. Luque, A. et al. (2003) J. Biol. Chem. 278:23656.
  12. Krampert, M. et al. (2005) J. Biol. Chem. 280:23844.
Long Name
A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 1
Entrez Gene IDs
9510 (Human)
Alternate Names
a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1; ADAM metallopeptidase with thrombospondin type 1 motif, 1; ADAM-TS 1; ADAMTS1; ADAM-TS1; ADAMTS-1; C3-C5; METH1; METH-1

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Citations for Human/Mouse ADAMTS1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Tau Protein Modulates Perineuronal Extracellular Matrix Expression in the TauP301L-acan Mouse Model
    Authors: Sophie Schmidt, Max Holzer, Thomas Arendt, Mandy Sonntag, Markus Morawski
    Biomolecules
  2. MYH9 facilitates autoregulation of adipose tissue depot development
    Authors: SY Cheung, M Sayeed, K Nakuluri, L Li, BJ Feldman
    JCI Insight, 2021-05-10;6(9):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. ADAMTS1 Supports Endothelial Plasticity of Glioblastoma Cells with Relevance for Glioma Progression
    Authors: O Serrano-Ga, C Peris-Torr, S Redondo-Ga, HG Asenjo, MDC Plaza-Calo, JL Fernandez-, JC Rodríguez-
    Biomolecules, 2020-12-31;11(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Extracellular Protease ADAMTS1 Is Required at Early Stages of Human Uveal Melanoma Development by Inducing Stemness and Endothelial-Like Features on Tumor Cells
    Authors: C Peris-Torr, MDC Plaza-Calo, R López-Domí, S Domínguez-, A Barrientos, P Carmona-Sá, JC Rodríguez-
    Cancers (Basel), 2020-03-27;12(4):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. ADAMTS-1 in abdominal aortic aneurysm
    Authors: E Vorkapic, M Folkesson, K Magnell, M Bohlooly-Y, T Länne, D Wågsäter
    PLoS ONE, 2017-06-01;12(6):e0178729.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  6. A glucocorticoid- and diet-responsive pathway toggles adipocyte precursor cell activity in vivo.
    Authors: Wong J, Krueger K, Costa M, Aggarwal A, Du H, McLaughlin T, Feldman B
    Sci Signal, 2016-10-25;9(451):ra103.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Stroma-derived but not tumor ADAMTS1 is a main driver of tumor growth and metastasis
    Authors: Rubén Fernández-Rodríguez, Francisco Javier Rodríguez-Baena, Estefanía Martino-Echarri, Carlos Peris-Torres, María del Carmen Plaza-Calonge, Juan Carlos Rodríguez-Manzaneque
    Oncotarget
  8. Galnt1 is required for normal heart valve development and cardiac function.
    Authors: Tian, E, Stevens, Sharon R, Guan, Yu, Springer, Danielle, Anderson, Stasia A, Starost, Matthew, Patel, Vyomesh, Ten Hagen, Kelly G, Tabak, Lawrence
    PLoS ONE, 2015-01-23;10(1):e0115861.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. ADAMTS1 inhibits lymphangiogenesis by attenuating phosphorylation of the lymphatic endothelial cell-specific VEGF receptor.
    Authors: Inagaki J, Takahashi K, Ogawa H, Asano K, Faruk Hatipoglu O, Cilek M, Obika M, Ohtsuki T, Hofmann M, Kusachi S, Ninomiya Y, Hirohata S
    Exp Cell Res, 2014-03-11;323(2):263-75.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. ADAMTS1, MPDZ, MVD, and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment
    Authors: Thashi Bharadwaj, Isabelle Schrauwen, Sakina Rehman, Khurram Liaqat, Anushree Acharya, Arnaud P. J. Giese et al.
    European Journal of Human Genetics

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