Human/Mouse ADAMTS15 Antibody Summary
Gly18-Cys682
Accession # Q8TE58
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse ADAMTS15 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, BG01V human embryonic stem cells, and mouse embryo tissue. PVDF Membrane was probed with 1 µg/mL of Human/Mouse ADAMTS15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5149) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ADAMTS15 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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ADAMTS15 in Human Breast Cancer Tissue. ADAMTS15 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human/Mouse ADAMTS15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5149) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hemotoxylin (blue). Specific staining was localized to stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAMTS15
ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) is a group of nineteen secreted zinc binding metalloproteases that is involved in development, inflammation, angiogenesis and several pathological conditions (1, 2). They are synthesized as zymogens which have a pro-domain that is removed by furin-like protein convertases. The mature secreted enzymes consist of catalytic, disintegrin-like, type I thrombospondin (TSP) motif, cysteine-rich, and spacer domains followed by zero to fourteen additional TSP motifs. Among ADAMTS proteases, ADAMTS1, -4, -5, -8, and -15 form a subfamily that possess aggrecanase activity (1 - 3). While ADAMTS4 and -5 (also known as aggrecanse-1 and -2, respectively) (4) have been well studied, the enzymatic activity of ADAMTS15 remains to be explored. It is suggested that ADAMTS15 may be involved in aggrecan degradation and breast cancer (5, 6). ADAMTS15 may also exhibit tumor suppressor activities since it contains genetic mutations in human colorectal carcinomas and restrains tumor growth and invasion in both in vitro and in vivo studies (7). The purified rhADAMTS15 is truncated and ends before the spacer domain.
- Porter, S. et al. (2005) Biochem. J. 386:15.
- Jones, G.C. and G.P. Riley (2005) Arthritis Res. Ther. 7:160.
- Nicholson, A.C. et al. (2005) BMC Evol. Biol. 5:11.
- Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
- Porter, S. et al. (2006) Intl. J. Cancer 118:1241.
- Kevorkian, L. et al. (2004) Arthritis Rheum. 50:131.
- Viloria, C.G. et al. (2009) Cancer Res. 69:4926.
Product Datasheets
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We used this antibody for developing a sandwich ELISA in combination with mAb (5149) and Protein std (5149-AD). This combination worked well and detected ADAMTD 15 efficiently in human serum and plasma samples.
