Human/Mouse Adenosine Deaminase/ADA Antibody Summary
Met1-Leu363
Accession # P00813
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Adenosine Deaminase/ADA by Western Blot. Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Adenosine Deaminase/ADA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7048) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Adenosine Deaminase/ ADA at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western Blot Shows Human Adenosine Deaminase/ADA Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Adenosine Deaminase/ADA knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Adenosine Deaminase/ADA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7048) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Adenosine Deaminase/ADA at approximately 45 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. New adjunct appears with knockout cell line.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Adenosine Deaminase/ADA
Adenosine Deaminase (ADA, adenosine aminohydrolase) is one of the key enzymes of purine nucleotide catabolism. It catalyses the hydrolytic deamination of adenosine and deoxy‑adenosine to inosine and deoxyinosine (1, 2). ADA is expressed in virtually all tissues and is expressed at high levels in T-lymphocytes. Adenosine Deaminase deficiency can cause a form of SCID (severe combined immunodeficiency) and lymphopenia in both B- and T-cell lineages (3, 4). ADA can be used as a sensitive diagnostic marker for tuberculous pleuritis (5). Although it is primarily a cytosolic enzyme, ADA is known to be a positive regulator of T-cell co‑activation due to its binding to CD26 at the cell surface. The interaction of ADA with CD26 regulates lymphocyte-epithelial cell adhesion (6).
- Wolfenden, R.V. et al. (1969) Biochemistry 6:2412.
- Lowenstein, J.M. (1972) Physiol. Rev. 52:382.
- Giblett, E.R. et al. (1972) Lancet 2:1067.
- Coleman, M.S. et al. (1978) J. Biol. Chem. 253:1619.
- Baba, K. et al. (2008) PLoS ONE 3:e2788.
- Gines, S. et al. (2002) Biochem. J. 361:203.
Product Datasheets
Citation for Human/Mouse Adenosine Deaminase/ADA Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Metabolite and thymocyte development defects in ADA-SCID mice receiving enzyme replacement therapy
Authors: FA Moretti, G Giardino, TCH Attenborou, AS Gkazi, BK Margetts, G la Marca, L Fairbanks, T Crompton, HB Gaspar
Scientific Reports, 2021-12-01;11(1):23221.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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