Human/Mouse AlphaA Crystallin/CRYAA Antibody

Catalog #: AF4848 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4848
AF4848-SP

Detection of Human/Mouse AlphaA Crystallin/CRYAA by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse AlphaA Crystallin/CRYAA Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse AlphaA Crystallin/CRYAA in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human CRYAB is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human AlphaA Crystallin/CRYAA
Met1-Ser173
Accession # P02489

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Simple Western

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse AlphaA Crystallin/CRYAA by Western Blot. Western blot shows lysates of mouse eye lens tissue. PVDF membrane was probed with 0.1 µg/mL Goat Anti-Human/Mouse AlphaA Crystallin/CRYAA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4848) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human CRYAA and CRYAB (5 ng/lane) were included. Bands for AlphaA Crystallin/CRYAA were detected at approximately 20 - 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western

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Detection of Mouse AlphaA Crystallin/CRYAA by Simple Western^TM. Simple Western lane view shows lysates of mouse eye lens tissue, loaded at 0.2 mg/mL. A specific band was detected for AlphaA Crystallin/CRYAA at approximately 28 kDa (as indicated) using 1 µg/mL of Goat Anti-Human/Mouse AlphaA Crystallin/CRYAA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4848) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AlphaA Crystallin/CRYAA

AlphaA Crystallin (CRYAA, also known as Crystallin alpha -A chain and HspB4) is a 21‑22 kDa member of the HSP20 family of proteins. It is found in lens epithelium where it noncovalently oligomerizes with CRY-alpha B to generate a transparent 350‑1000 kDa alpha -Crystallin protein complex. Human CRYAA is 173 amino acids (aa) in length. There is a alpha -Crystallin Hsp domain over aa 63‑145. Multiple posttranslational modifications may exist. The C-terminal Ser and the N-terminal MetAspVal is occasionally cleaved. There is also phosphorylation at Ser45 and 122, O-linked GlcNAc modification at Ser162, deamidation at Asn101, and acetylation at Lys70. Full‑length human CRYAA is 54% aa identical to CRY-AlphaB, and 95% aa identical to mouse CRYAA.

Entrez Gene IDs

1409 (Human); 12954 (Mouse)

Alternate Names

AlphaA Crystallin; CRYA1; CRYA1crystallin, alpha-1; CRYAA; crystallin, alpha A; Heat shock protein beta-4; HSPB4; HSPB4alpha-crystallin A chain; human alphaA-crystallin (CRYA1), 10HspB4