Human/Mouse ATN1 Antibody

Catalog #: AF6567 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6567
AF6567-SP

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Human/Mouse ATN1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse ATN1 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ATN1
Met1-Gln100
Accession # P54259

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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ATN1 in Human Brain. ATN1 was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Sheep Anti-Human/Mouse ATN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6567) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry

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Detection of ATN1 in Mouse Liver. ATN1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Sheep Anti-Human/Mouse ATN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6567) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATN1

ATN1 (Atrophin-1; also DRPLA protein) is a 190‑200 kDa member of the Atrophin family of proteins that is cleaved into 120‑150 kDa fragments. ATN1 is a ubiquitously expressed transcriptional coactivator. Human ATN1 is 1185 amino acids (aa) in length. It contains multiple motifs, including an NLS (aa 16-32), interspersed poly-Pro, poly-Ser and poly-His regions (aa 376-707), two RE (ArgGlu) repeats (aa 816-934), and an NES (aa 1033-1042). There are at least 17 utilized phosphorylation sites and one acetylated Lys. ATN1 is most characterized by a poly-Gln region between aa 484-497. Normally, there are about 20 consecutive Gln residues, but this number may be increased to more than 70 in pathologic conditions. Proteolytic cleavage generates large C-terminal fragments of 120-150 kDa size. These are unlikely to contain the NLS, and thus are typically cytosolic. ATN1 is suggested to form heterodimers with full-length ATN2/RERE, thus generating a transcriptional repressor. There are multiple potential isoforms. One shows an alternative start site at Met527, while others differ in the number of glutamines in the poly-Gln region. Over aa 1-100, human ATN1 shares 94% aa identity with mouse ATN1.

Long Name

Atrophin 1

Entrez Gene IDs

1822 (Human); 13498 (Mouse); 29515 (Rat)

Alternate Names

ATN1; atrophin 1; atrophin-1; B37; D12S755E; dentatorubral-pallidoluysian atrophy (atrophin-1); Dentatorubral-pallidoluysian atrophy protein; DRPLA; DRPLANOD; HRS; NOD