Human/Mouse BLIMP1/PRDM1 Antibody

Catalog #: AF3608
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3608
AF3608-SP

Detection of Human BLIMP1/PRDM1 by Western Blot.

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All BLIMP1/PRDM1 Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse BLIMP1/PRDM1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse BLIMP1/PRDM1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BLIMP1/PRDM1
Leu687-Pro789
Accession # AAO45623

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human BLIMP1/PRDM1 by Western Blot. Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse BLIMP1/PRDM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3608) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for BLIMP1/PRDM1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BLIMP1/PRDM1

BLIMP-1 is a 97 kDa multidomain transcription repressor that has a SET domain in its N-terminal region and four C2H2-type zinc fingers in its C-terminal regions. It promotes terminal differentiation of B cells into plasma cells and also has an important role in primordial germ cell specification. An alternative splice variant that lacks 101 N-terminal amino acid (aa) residues of full-length BLIMP1 has been reported. Over the region used as immunogen, human and mouse BLIMP1 share 89% aa sequence identity.

Long Name

B Lymphocyte Induced Maturation Protein

Entrez Gene IDs

639 (Human); 12142 (Mouse); 309871 (Rat)

Alternate Names

Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP1; BLIMP-1; BLIMP1MGC118925; blmp-1; B-lymphocyte-induced maturation protein 1; MGC118922; MGC118923; Positive regulatory domain I-binding factor 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI-BF1MGC118924; PRDI-binding factor 1; PRDI-binding factor-1; PRDM1; PR-domain zinc finger protein 1; ZNFPR1A1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse BLIMP1/PRDM1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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A role for Lin28 in primordial germ-cell development and germ-cell malignancy.
Authors: West JA, Viswanathan SR, Yabuuchi A, Cunniff K, Takeuchi A, Park IH, Sero JE, Zhu H, Perez-Atayde A, Frazier AL, Surani MA, Daley GQ
Nature, 2009-07-05;460(7257):909-13.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Combined whole-organ imaging at single-cell resolution and immunohistochemical analysis of prostate cancer and its liver and brain metastases
Authors: J Taranda, G Mathew, K Watrud, N El-Amine, MF Lee, C Elowsky, A Bludova, S Escobar Av, DG Nowak, TL Wee, JE Wilkinson, LC Trotman, P Osten
Cell Reports, 2021-11-16;37(7):110027.