Human/Mouse BLMH/Bleomycin Hydrolase Antibody

Catalog #: AF6200 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6200
AF6200-SP

Detection of Human and Mouse BLMH by Western Blot.

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Human/Mouse BLMH/Bleomycin Hydrolase Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse BLMH in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BLMH
Ser2-Glu455
Accession # Q13867

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse BLMH by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and Neuro-2A mouse neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Human BLMH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6200) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BLMH at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BLMH/Bleomycin Hydrolase

Bleomycin Hydrolase (BLMH) is a cysteine peptidase of the papain superfamily. It is named for its ability to hydrolyze the antitumor agent bleomycin and inactivate it (1). It has a papain-like catalytic triad (Cys-His-Asp) with optimum activity at neutral pH. In mammals it is expressed ubiquitously in all types of tissues and its expression is up‑regulated in many tumors. It is present in the cytoplasm as homohexameric protein of approximately 300 kDa. In addition to its aminopeptidase activity, it has homocysteine-thiolactonase activity. The normal physiological function of BLMH is not clear. BLMH inactivates bleomycin, a glycopeptide anticancer agent, by deaminating it (2). BLMH has been suggested to play a role in the generation of MHC class I-presented peptides (3 ‑ 4). Diminished BLMH activity may contribute to the pathology of Alzheimer’s disease (AD) (5 ‑ 6). It is inhibited by cysteine protease inhibitors such as N-ethylmaleimide, iodoacetamide, para-hydroxymercuribenzoate, and E-64.

References

Joshua-Tor, L. and S. A. Johnson (2004) in Handbook of Proteolytic Enzymes, Barrett, A. J. et al. eds. pp. 1197.
Schwartz, D. R. et al. (1999) Proc. Natl. Acad. Sci. USA, 96:4680.
Kim, E. et al. (2009) J. Immunol. 183:7379.
Towne, C. F. et al. (2007) J. Immunol. 178:6923.
Suszynska, J. et al. (2010) J. Alzheimers Dis. 19:1177.
Lefterov, I. M. et al. (2000) FASEB J. 14:1837.