Human/Mouse Bub3 Antibody

Catalog #: AF5706 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5706
AF5706-SP

Detection of Human/Mouse Bub3 by Western Blot.

1 Image

All Bub3 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Bub3 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Bub3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human Bub1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Bub3
Met1-Thr328
Accession # O43684

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human/Mouse Bub3 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Bub3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5706) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Bub3 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bub3

Bub3 (Budding uninhibited by benzimidazoles 3 homolog; also Mitotic checkpoint protein Bub3) is a 35‑40 kDa member of the WD repeat Bub3 family of molecules. It plays a key role in chromosome segregation by serving as a component of the mitotic checkpoint complex. This complex inhibits Cdc20, and E3 ubiquitin ligase that initiates the metaphase to anaphase transition, until microtubules are properly aligned with, and bound to, chromatid kinetochores. Human Bub3 is 328 amino acids (aa) in length. It contains five WD40 repeats (aa 5‑43, 46‑83, 86‑124, 128‑163 and 223‑262) plus a phosphorylation site at Ser211 and a Lys at # 216 that may form an interchain crosslink with Gly. There are two splice variants that show two or four aa deletion over the four C‑terminal amino acids. Full‑length human and mouse Bub3 share 99% aa identity.

Long Name

BUB3 Budding Uninhibited By Benzimidazoles 1 Homolog

Entrez Gene IDs

9184 (Human); 12237 (Mouse)

Alternate Names

BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog; BUB3 budding uninhibited by benzimidazoles 3 homolog; Bub3; BUB3L; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog (yeast); hBUB3; mitotic checkpoint component; mitotic checkpoint protein BUB3