Human/Mouse Cadherin Pan Specific Antibody

Detection of Cadherin in Human Liver via Multiplex Immunofluorescence staining on COMET™ Cadherin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human/Mouse Cadherin Pan Specific Monoclonal Antibody (MAB18385) at 15 μg/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of Cadherin in Human Colon via Multiplex Immunofluorescence staining on COMET™ Cadherin was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human/Mouse Cadherin Pan Specific Monoclonal Antibody (MAB18385) at 15 μg/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of Cadherin in Human Colon Cancer via Multiplex Immunofluorescence staining on COMET™ Cadherin was detected in immersion fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human/Mouse Cadherin Pan Specific Monoclonal Antibody (MAB18385) at 15 μg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of Human and Mouse Cadherin by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, MCF-7 human breast cancer cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Cadherin Pan Specific Monoclonal Antibody (Catalog # MAB18385) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). Specific bands were detected for Cadherin at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cadherin in Human Cystadenocarcinoma. Cadherin was detected in immersion fixed paraffin-embedded sections of human papillary serous cystadenocarcinoma of the colon using Mouse Anti-Human/Mouse Cadherin Pan Specific Monoclonal Antibody (Catalog # MAB18385) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human Cadherins by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line mock transfected or transfected with human E-Cadherin and NS0 mouse myeloma cell line transfected with Human P-Cadherin, N-Cadherin, or mock transfected. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Cadherin Pan Specific Monoclonal Antibody (Catalog # MAB18385) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Cadherin at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.