Human/Mouse Carbonic Anhydrase VIII/CA8 Antibody

Catalog #: MAB2187 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2187
MAB2187-SP

Detection of Human and Mouse Carbonic Anhydrase VIII/CA8 by Western Blot.

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All Carbonic Anhydrase VIII/CA8 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Carbonic Anhydrase VIII/CA8 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human Carbonic Anhydrase VIII/CA8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CA1, 2, 3, 4, 9, 10, 12, 13, or 14 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 308320

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Carbonic Anhydrase VIII/CA8
Ala2-Gln290
Accession # P35219

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Carbonic Anhydrase VIII/CA8 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue and mouse brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Carbonic Anhydrase VIII/CA8 Monoclonal Antibody (Catalog # MAB2187) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Carbonic Anhydrase VIII/CA8 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase VIII/CA8

Carbonic Anhydrase VIII (CA8), also called CA‑related protein (CARP), is a cytosolic protein without CA activity (i.e., the reversible hydration of CO₂) due to point mutations in the zinc-binding site (1, 2). Nevertheless, it has esterase activity. CA8 is expressed exclusively in Purkinje cells of the cerebellum, where it binds inositol 1,4,5-triphosphate receptor type 1 (3). CA8 overexpression in human colorectal cancer and non-small cell lung cancer indicates that it plays a role in the process of invasion in these types of malignancy (4, 5). The 289 amino acid rhCA8 has a predicted molecular mass of approximately 33 kDa. It shares 98% amino acid identity with mouse, rat and bovine CA8 and 78% with Xenopus.

References

Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Sjoblom, J. et al. (1996) FEBS Lett. 398: 322.
Hirota, J. et al. (2003) Biochem. J. 372: 435.
Miyaji, E. et al. (2003) J. Pathol. 201: 37.
Lu, S.H. et al. (2004) Lung Cancer 44: 273.

Entrez Gene IDs

767 (Human)

Alternate Names

CA8; CALS; CALScarbonate dehydratase; CAMRQ3; Carbonic Anhydrase VIII; carbonic anhydrase VIIIMGC99509; carbonic anhydrase-like sequence; carbonic anhydrase-related protein; CARP; CARPCA-related protein; CA-VIII; MGC120502