Human/Mouse Carbonic Anhydrase VIII/CA8 Antibody

Catalog #: AF2187 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2187
AF2187-SP

Detection of Human and Mouse Carbonic Anhydrase VIII/CA8 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Carbonic Anhydrase VIII/CA8 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human Carbonic Anhydrase VIII/CA8 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) CA2 and rhCA3 is observed and less than 1% cross-reactivity with rhCA1, rhCA4, and rhCA9 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Carbonic Anhydrase VIII/CA8
Ala2-Gln290
Accession # P35219

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

5 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase VIII (Catalog # 2187-CA), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Carbonic Anhydrase VIII/CA8 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue and mouse brain (cerebellum) tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Carbonic Anhydrase VIII/CA8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2187) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase VIII/CA8 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human and Mouse Carbonic Anhydrase VIII/CA8 by Simple Western^TM. Simple Western lane view shows lysates of human brain (cerebellum) tissue and mouse brain (cerebellum) tissue, loaded at 0.2 mg/mL. Specific bands were detected for Carbonic Anhydrase VIII/CA8 at approximately 41 and 50 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse Carbonic Anhydrase VIII/CA8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2187) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase VIII/CA8

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. CA8, also called CA‑related protein (CARP), is a cytosolic protein without CA activity (i.e., the reversible hydration of CO₂) due to point mutations in the zinc-binding site (2). Nevertheless, it has esterase activity described in the Activity Assay Protocol. CA8 is expressed exclusively in Purkinje cells of the cerebellum, where it binds inositol 1,4,5‑triphosphate receptor type 1 (3). CA8 overexpression in human colorectal cancer and non-small cell lung cancer indicates that it plays a role in the process of invasion in these types of malignancy (4, 5).

References

Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Sjoblom, J. et al. (1996) FEBS Lett. 398: 322.
Hirota, J. et al. (2003) Biochem. J. 372: 435.
Miyaji, E. et al. (2003) J. Pathol. 201: 37.
Lu, S.H. et al. (2004) Lung Cancer 44: 273.

Entrez Gene IDs

767 (Human)

Alternate Names

CA8; CALS; CALScarbonate dehydratase; CAMRQ3; Carbonic Anhydrase VIII; carbonic anhydrase VIIIMGC99509; carbonic anhydrase-like sequence; carbonic anhydrase-related protein; CARP; CARPCA-related protein; CA-VIII; MGC120502