Human/Mouse Cathepsin B Antibody

Catalog #: MAB965
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB965
MAB965-SP

All Cathepsin B Antibodies

Product Details

Citations (5)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Cathepsin B Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects the pro and active forms of human and mouse Cathepsin B in direct ELISAs and Western blots. Does not cross‑react with recombinant human Cathepsin C, L, L2, O, S, Z, recombinant mouse Cathepsin C, H, L, or Z.

Source

Monoclonal Rat IgG₁ Clone # 173317

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin B
His18-Phe339
Accession # P10605

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Cathepsin B (Catalog # 953-CY)
Recombinant Mouse Cathepsin B (Catalog # 965-CY)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin B

Cathepsin B is a cysteine protease that is known to process a number of proteins. It is up-regulated or redistributed in human and animal tumors.

Entrez Gene IDs

1508 (Human); 13030 (Mouse)

Alternate Names

APP secretase; APPS; Cathepsin B; Cathepsin B1; CPSBamyloid precursor protein secretase; CTSB; cysteine protease; EC 3.4.22; EC 3.4.22.1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse Cathepsin B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Mammary mechanobiology - investigating roles for mechanically activated ion channels in lactation and involution
Authors: Teneale A. Stewart, Katherine Hughes, Alexander J. Stevenson, Natascia Marino, Adler L. Ju, Michael Morehead et al.
Journal of Cell Science
C9orf72 and smcr8 mutant mice reveal MTORC1 activation due to impaired lysosomal degradation and exocytosis
Authors: Q Shao, M Yang, C Liang, L Ma, W Zhang, Z Jiang, J Luo, JK Lee, C Liang, JF Chen
Autophagy, 2019-12-26;0(0):1-16.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
Induction of cathepsin B by the CXCR3 chemokines CXCL9 and CXCL10 in human breast cancer cells
Authors: H Bronger, A Karge, T Dreyer, D Zech, S Kraeft, S Avril, M Kiechle, M Schmitt
Oncol Lett, 2017-04-05;13(6):4224-4230.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model.
Authors: Wendt W, Schulten R, Stichel CC, Lubbert H
J. Neurochem., 2009-07-17;110(6):1931-41.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
CD14 release induced by P2X7 receptor restricts inflammation and increases survival during sepsis
Authors: Cristina Alarcón-Vila, Alberto Baroja-Mazo, Carlos de Torre-Minguela, Carlos M Martínez, Juan J Martínez-García, Helios Martínez-Banaclocha et al.
eLife