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Human/Mouse Cited-2 Antibody

Catalog #: AF5005
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5005
AF5005-SP
Detection of Human and Mouse Cited‑2 by Western Blot.
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Citations (5)
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Human/Mouse Cited-2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse Cited-2 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Cited-2
Met1-Cys269
Accession # O35740
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
3-15 µg/mL
See below
Immunocytochemistry
1-10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Cited-2 antibody by Western Blot. View Larger

Detection of Human and Mouse Cited‑2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Cited-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cited-2 at approximately 30-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Cited-2 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Cited‑2 in HeLa Human Cell Line. Cited-2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse Cited-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Cited-2 antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Cited‑2 in C2C12 Mouse Cell Line. Cited-2 was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Human/Mouse Cited-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Cited-2 antibody in NIH-3T3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Cited‑2 in NIH‑3T3 Mouse Cell Line. Cited-2 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Sheep Anti-Human/Mouse Cited-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Cited-2 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Cited‑2 in Human Breast Cancer Tissue. Cited-2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human/Mouse Cited-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5005) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cited-2

Cited-2 is a 270 amino acid protein that functions by binding to the CH1 region of CREB binding protein (CBP)/p300. This binding interferes with certain transcription factors like HIF1 alpha and STAT2 blocking their ability to bind and interact with CBP/p300. Cited-2 knockout mice die due to defects in heart and neural tube development. Mutations in Cited-2 have been reported in patients with congenital heart defects. It appears Cited-2 modulates apoptosis by a p53 independent pathway whereby the details still need to be elucidated.

Long Name
Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2
Entrez Gene IDs
10370 (Human); 17684 (Mouse); 114490 (Rat)
Alternate Names
Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain2,cbp/p300-interacting transactivator 2; Cited2; Cited-2; MRG-1; MRG1melanocyte-specific gene 1-related gene 1; MSG1-related gene 1; MSG-related protein 1; P35srj

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Citations for Human/Mouse Cited-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Fetal and trophoblast PI3K p110 alpha have distinct roles in regulating resource supply to the growing fetus in mice
    Authors: Jorge López-Tello, Vicente Pérez-García, Jaspreet Khaira, Laura C Kusinski, Wendy N Cooper, Adam Andreani et al.
    eLife
  2. Cited2, a transcriptional modulator protein, regulates metabolism in murine embryonic stem cells.
    Authors: Li Q, Hakimi P, Liu X, Yu W, Ye F, Fujioka H, Raza S, Shankar E, Tang F, Dunwoodie S, Danielpour D, Hoppel C, Ramirez-Bergeron D, Qu C, Hanson R, Yang Y
    J Biol Chem, 2013-11-21;289(1):251-63.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ChIP
  3. Cited2 gene controls pluripotency and cardiomyocyte differentiation of murine embryonic stem cells through Oct4 gene.
    Authors: Li Q, Ramirez-Bergeron D, Dunwoodie S, Yang Y
    J Biol Chem, 2012-07-03;287(34):29088-100.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP, Western Blot
  4. HIF-1 alpha deletion partially rescues defects of hematopoietic stem cell quiescence caused by Cited2 deficiency.
    Authors: Du J, Chen Y, Li Q, Han X, Cheng C, Wang Z, Danielpour D, Dunwoodie SL, Bunting KD, Yang YC
    Blood, 2012-02-02;119(12):2789-98.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  5. CITED2 attenuates macrophage recruitment concordant with the downregulation of CCL20 in breast cancer cells
    Authors: Swaathi Jayaraman, Michele Doucet, Scott L. Kominsky
    Oncology Letters

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