Human/Mouse Cleaved Caspase-3 Alexa Fluor® 488-conjugated Antibody Summary
CRGTELDCGIETD
Accession # U26943
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Cleaved Caspase‑3 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line untreated (open histogram) or treated with 3 µM Staurosporine for 3 hours (filled histogram) was stained with Rabbit Anti-Human/Mouse Caspase-3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC835G, filled histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol. View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Caspase-3
Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.
Product Datasheets
Citations for Human/Mouse Cleaved Caspase-3 Alexa Fluor® 488-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Apoptosis Induction in HepG2 and HCT116 Cells by a Novel Quercetin-Zinc (II) Complex: Enhanced Absorption of Quercetin and Zinc (II)
Authors: Nakamura, M;Urakawa, D;He, Z;Akagi, I;Hou, DX;Sakao, K;
International journal of molecular sciences
Species: Human
Sample Types: Whole Cells
Applications: ICC -
EMAPII Monoclonal Antibody Ameliorates Influenza A Virus-Induced Lung Injury
Authors: H Lu, S Chelvanamb, C Poirier, J Saliba, KL March, M Clauss, NV Bogatcheva
Mol. Ther., 2018-06-15;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Chemotherapy-induced differential cell cycle arrest in B cell lymphomas affects their sensitivity to Wee1 inhibition
Authors: X Wang, Z Chen, AK Mishra, A Silva, W Ren, Z Pan, JH Wang
Haematologica, 2017-12-07;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
STAT3 Activation-Induced Fatty Acid Oxidation in CD8+ T Effector Cells Is Critical for Obesity-Promoted Breast Tumor Growth
Authors: Chunyan Zhang, Chanyu Yue, Andreas Herrmann, Jieun Song, Colt Egelston, Tianyi Wang et al.
Cell Metabolism
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