Home / Caspase-3 / Human/Mouse Cleaved Caspase-3 (Asp175) Antibody

Human/Mouse Cleaved Caspase-3 (Asp175) Antibody

Catalog #: MAB835
99 Citations 8 Reviews Datasheet / COA / SDS
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MAB835
MAB835-SP
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Detection of Human and Mouse Cleaved Caspase‑3 (Asp175) by Western Blot.
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Human/Mouse Cleaved Caspase-3 (Asp175) Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Caspase-3 cleaved at Asp175. No cross-reactivity was detected with the full-length procaspase-3 or other caspases.
Source
Monoclonal Rabbit IgG Clone # 269518
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH-conjugated human Caspase-3 synthetic peptide
CRGTELDCGIETD
Accession # U26943
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
0.3-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Cleaved Caspase‑3 (Asp175) antibody by Western Blot. View Larger

Detection of Human and Mouse Cleaved Caspase‑3 (Asp175) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line untreated (-) or treated (+) with 1 µM staurosporine (STS) for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Cleaved Caspase-3 (Asp175) Monoclonal Antibody (MAB835), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cleaved Caspase-3 (Asp175) at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry Caspase-3 antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC). View Larger

Caspase‑3 in Jurkat Human Cell Line. Caspase-3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with staurosporin using Human/Mouse Cleaved Caspase-3 (Asp175) Monoclonal Antibody (Catalog # MAB835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry Caspase-3 antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Caspase‑3 in Human Colon Cancer Tissue. Caspase-3 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Rabbit Anti-Human/Mouse Cleaved Caspase-3 (Asp175) Monoclonal Antibody (Catalog # MAB835) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Intracellular Staining by Flow Cytometry Detection of Cleaved Caspase-3 antibody in Jurkat Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Cleaved Caspase‑3 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line untreated (open histogram) or treated with 3 µM Staurosporine for 3 hours (filled histogram) was stained with Rabbit Anti-Human/Mouse Caspase-3 Monoclonal Antibody (Catalog # MAB835, filled histogram) followed by anti-Rabbit IgG FITC-conjugated secondary antibody (Catalog # F0112). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol. View our protocol for Staining Intracellular Molecules.

Immunocytochemistry/ Immunofluorescence Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence Caspase-3 activation and actin cytoskeletal organization in melanoma A375 cells cultured in the presence of PFII.Cells were grown on glass coverslips in the presence of 65, 130, 260 µM or 1 µM staurosporine (STS). (A–E) Active caspase-3 was visualized with anti-active caspase-3 antibody followed by a FITC-conjugated secondary antibody (green). (F–J) Actin was visualized using laser scanning confocal microscope (LSCM) after staining with Alexa Fluor 568 - conjugated phalloidin (red). Scale bar - 50 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0057991), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Caspase-3 by Western Blot View Larger

Detection of Mouse Caspase-3 by Western Blot Epidermis-specific deletion of Rac1 increases UV-light-induced keratinocyte apoptosis in vivo. (a) H/E staining of UV-irradiated skin of Rac1 fl/fl and Rac1-EKO mice at 12 h after UV-irradiation. Black arrows indicate sunburn cells. (b) Graph shows the percentage number of sunburn cells at 12 h with (red bars) or without (blue bars) UV-irradiation in Rac1 fl/fl (n=4) and Rac1-EKO (n=5) mice. The percentage of sunburn cells within the epidermis after UV-irradiation in Rac1 fl/fl mice was 3.4%, whereas in Rac1-EKO mice it was 8.6% of total epidermal keratinocytes. Non-irradiated samples showed <1% sunburn cells in both the genotypes. (c) Immunostainings against cleaved caspase-3 (green) of UV-irradiated skin of Rac1 fl/fl and Rac1-EKO mice at 12 h after UV-irradiation. Nuclei are stained in blue. Scale bar=100 μm. (d) Western blot analysis of cleaved caspase-3 from epidermal lysates of untreated (no UV) and UV-light treated (UV) Rac1 fl/fl and Rac1-EKO mice. Non-irradiated controls showed no bands for cleaved capsase-3, whereas samples of irradiated epidermis showed cleaved caspase-3-specific bands at ~25 kDa and 23 kDa. Numbers on the left denote molecular weights in kDa. (e) Graph shows densitometry analysis of western blot in (d). Error bars show S.D. Asterisks show P-value<0.001 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28277539), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence Apoptotic markers in PFII treated NHDFs.(A–C) [Ca2+]i fluorescence visualizations in cells as revealed by using an LSCM with fluorescent probe Fluo3/AM, scale bar - 100 µm. (D–F) Externalized phosphatidylserine by annexin V-fluorescein binding after PFII treatment, scale bar - 100 µm. Third (G–I) and fourth (J–L) panels show actin cytoskeletal organization and caspase-3 activation. Scale bar - 50 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0057991), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Caspase-3 by Western Blot View Larger

Detection of Mouse Caspase-3 by Western Blot Rac1 deficiency increases sensitivity towards UV-light-induced keratinocyte apoptosis in vitro. (a and b) Western blot analysis of cleaved caspase-3 from Rac1 fl/fl and Rac1-EKO cultured keratinocytes at 6 h (a) and at 12 h (b) with (UV) or without (no UV) UV-irradiation. (c) Densitometry analysis of cleaved caspase-3 at 6 h after UV-irradiation from Rac1 fl/fl and Rac1-EKO keratinocytes. (d) Quantification of CPDs by CPD ELISA carried out from genomic DNA isolated from Rac1 fl/fl and Rac1-EKO mouse primary keratinocytes immediately after UV-irradiation. Error bars show S.D. Asterisks show P-value<0.01 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28277539), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Caspase-3 by Western Blot View Larger

Detection of Mouse Caspase-3 by Western Blot Increase in UV-light-induced apoptosis in Rac1-deficient keratinocytes requires activation of caspase-8. (a) Western blot analysis of cleaved caspase-8 from Rac1 fl/fl and Rac1-EKO cultured keratinocytes at 6 h with (UV) or without (no UV) UV-irradiation. (b) Densitometry analysis of fold change of cleaved caspase-8 in (a) normalized to GAPDH in Rac1 fl/fl and Rac1-EKO samples after UV-irradiation. (c) Western blot analysis of cleaved caspase-8 from CPDPL/Rac1-EKO epidermal lysates from mice kept in the dark or under the photoreactivtion lamp (PR). (d) Densitometric analysis of cleaved caspase-8 western blots in c. Error bars show S.D. (e) Western blot analysis of cleaved caspase-3 from wild-type (WT) and TNF receptor-1-deficient (TNFR-1 KO) cultured keratinocytes at 6 h with (UV) or without (no UV) UV-irradiation. (f) Western blot analysis of cleaved caspase-3 from TNFR-1 KO cultured keratinocytes incubated with DMSO or Rac1 inhibitor (EHT 1864) at 6 h with (UV) or without (no UV) UV-irradiation. GAPDH is used as a loading control. Numbers on the left denote molecular weights in kDa. * and *** represent P-value<0.05 and <0.001, respectively Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28277539), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Rat Caspase-3 by Western Blot View Larger

Detection of Rat Caspase-3 by Western Blot Western blot analysis of high-mobility group box-1 (HMGB1), brain-derived neurotrophic factor (BDNF), synaptophysin, and cleaved caspase-3 in rat retinas. There is a significant increase in the expression of HMGB1 and cleaved caspase-3 and a significant decrease in the expression of BDNF and synaptophysin in the retinas of diabetic rats (D) compared with the nondiabetic control rats (N). Each experiment was repeated 2 to 3 times with fresh samples (n = 6). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23766563), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence BFSE decreases macrophage viability. THP-1 macrophage, or MDM cells, were exposure to air control or 1%, 2.5%, 5%, 10% BFSE or 10% CSE for 24 h. LDH from THP-1 macrophage or MDM cells was measured in supernatants after 24 h (n = 4) (A). THP-1 macrophage expression of Bcl-2 and PARP was assessed by western blot (B), band densitometry analysis of Bcl-2 (C) and PARP (D) was performed, data is a representation of three independent experiments, and was baselined to the air treated control sample, and normalized to beta -actin expression. Error bars represent 95% confidence intervals. Representative confocal images of active caspase-3 (E) and PAR (F), in THP-1 macrophage with quantitative MFI measurement of active caspase-3 (G) and PAR (H). (n = 4) *p < 0.05, **p < 0.01. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30194323), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human Caspase-3 by Western Blot View Larger

Detection of Human Caspase-3 by Western Blot Rac1 deficiency increases sensitivity towards UV-light-induced keratinocyte apoptosis in vitro. (a and b) Western blot analysis of cleaved caspase-3 from Rac1 fl/fl and Rac1-EKO cultured keratinocytes at 6 h (a) and at 12 h (b) with (UV) or without (no UV) UV-irradiation. (c) Densitometry analysis of cleaved caspase-3 at 6 h after UV-irradiation from Rac1 fl/fl and Rac1-EKO keratinocytes. (d) Quantification of CPDs by CPD ELISA carried out from genomic DNA isolated from Rac1 fl/fl and Rac1-EKO mouse primary keratinocytes immediately after UV-irradiation. Error bars show S.D. Asterisks show P-value<0.01 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28277539), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence Caspase-3 activation and actin cytoskeletal organization in melanoma A375 cells cultured in the presence of PFII.Cells were grown on glass coverslips in the presence of 65, 130, 260 µM or 1 µM staurosporine (STS). (A–E) Active caspase-3 was visualized with anti-active caspase-3 antibody followed by a FITC-conjugated secondary antibody (green). (F–J) Actin was visualized using laser scanning confocal microscope (LSCM) after staining with Alexa Fluor 568 - conjugated phalloidin (red). Scale bar - 50 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0057991), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence Caspase-3 activation and actin cytoskeletal organization in melanoma A375 cells cultured in the presence of PFII.Cells were grown on glass coverslips in the presence of 65, 130, 260 µM or 1 µM staurosporine (STS). (A–E) Active caspase-3 was visualized with anti-active caspase-3 antibody followed by a FITC-conjugated secondary antibody (green). (F–J) Actin was visualized using laser scanning confocal microscope (LSCM) after staining with Alexa Fluor 568 - conjugated phalloidin (red). Scale bar - 50 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0057991), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence Caspase-3 activation and actin cytoskeletal organization in melanoma A375 cells cultured in the presence of PFII.Cells were grown on glass coverslips in the presence of 65, 130, 260 µM or 1 µM staurosporine (STS). (A–E) Active caspase-3 was visualized with anti-active caspase-3 antibody followed by a FITC-conjugated secondary antibody (green). (F–J) Actin was visualized using laser scanning confocal microscope (LSCM) after staining with Alexa Fluor 568 - conjugated phalloidin (red). Scale bar - 50 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0057991), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Rat Caspase-3 by Western Blot View Larger

Detection of Rat Caspase-3 by Western Blot Western blot analysis of rat retinas. Intravitreal administration of high-mobility group box-1 (HMGB1) induced a significant upregulation of the expression of HMGB1 and cleaved caspase-3 and a significant downregulation of the expression of brain-derived neurotrophic factor (BDNF) and synaptophysin compared with intravitreal administration of phosphate buffer saline (PBS). Each experiment was repeated 2 to 3 times with fresh samples (n = 6). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23766563), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence Apoptotic markers in PFII treated NHDFs.(A–C) [Ca2+]i fluorescence visualizations in cells as revealed by using an LSCM with fluorescent probe Fluo3/AM, scale bar - 100 µm. (D–F) Externalized phosphatidylserine by annexin V-fluorescein binding after PFII treatment, scale bar - 100 µm. Third (G–I) and fourth (J–L) panels show actin cytoskeletal organization and caspase-3 activation. Scale bar - 50 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0057991), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence Apoptotic markers in PFII treated NHDFs.(A–C) [Ca2+]i fluorescence visualizations in cells as revealed by using an LSCM with fluorescent probe Fluo3/AM, scale bar - 100 µm. (D–F) Externalized phosphatidylserine by annexin V-fluorescein binding after PFII treatment, scale bar - 100 µm. Third (G–I) and fourth (J–L) panels show actin cytoskeletal organization and caspase-3 activation. Scale bar - 50 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0057991), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence Caspase-3 activation and actin cytoskeletal organization in melanoma A375 cells cultured in the presence of PFII.Cells were grown on glass coverslips in the presence of 65, 130, 260 µM or 1 µM staurosporine (STS). (A–E) Active caspase-3 was visualized with anti-active caspase-3 antibody followed by a FITC-conjugated secondary antibody (green). (F–J) Actin was visualized using laser scanning confocal microscope (LSCM) after staining with Alexa Fluor 568 - conjugated phalloidin (red). Scale bar - 50 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0057991), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Caspase-3 by Western Blot View Larger

Detection of Mouse Caspase-3 by Western Blot Increase in UV-light-induced apoptosis in Rac1-deficient keratinocytes requires activation of caspase-8. (a) Western blot analysis of cleaved caspase-8 from Rac1 fl/fl and Rac1-EKO cultured keratinocytes at 6 h with (UV) or without (no UV) UV-irradiation. (b) Densitometry analysis of fold change of cleaved caspase-8 in (a) normalized to GAPDH in Rac1 fl/fl and Rac1-EKO samples after UV-irradiation. (c) Western blot analysis of cleaved caspase-8 from CPDPL/Rac1-EKO epidermal lysates from mice kept in the dark or under the photoreactivtion lamp (PR). (d) Densitometric analysis of cleaved caspase-8 western blots in c. Error bars show S.D. (e) Western blot analysis of cleaved caspase-3 from wild-type (WT) and TNF receptor-1-deficient (TNFR-1 KO) cultured keratinocytes at 6 h with (UV) or without (no UV) UV-irradiation. (f) Western blot analysis of cleaved caspase-3 from TNFR-1 KO cultured keratinocytes incubated with DMSO or Rac1 inhibitor (EHT 1864) at 6 h with (UV) or without (no UV) UV-irradiation. GAPDH is used as a loading control. Numbers on the left denote molecular weights in kDa. * and *** represent P-value<0.05 and <0.001, respectively Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28277539), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Human/Mouse Cleaved Caspase-3 (Asp175) Antibody by Western Blot View Larger

Detection of Mouse Human/Mouse Cleaved Caspase-3 (Asp175) Antibody by Western Blot Gamma herpesvirus 68 ( gamma HV68) replicates in alveolar epithelial cells (AECs). (A) Wild-type mice were infected with 5 × 104 PFU gamma HV68 on day 0. On day 7 after infection, frozen sections were prepared, and stained with a rabbit polyclonal antisera against gamma HV68, or with non-immune rabbit sera as control. The goat anti-rabbit secondary was linked to alkaline phosphatase. Vivid replication of gamma HV68 is visible in alveolar lining cells (original magnification × 100). Sections shown are representative of four mice examined. (B) AECs were isolated from lungs of Balb/c or TLR-9-/- mice treated with bleomycin plus gamma HV68 on day 21, and were cultured on fibronectin-coated slides (TiterTek). Sections were stained with antibodies (M30 Cytodeath), and the number of positive cells per high power field (HPF; ×400) were calculated (n = 30 HPF per genotype). (C) AECs were isolated from Balb/c or TLR-9-/- mice and cells were infected in vitro with 0.01 or 0.001 PFU gamma HV68 for 48 hours. Cell lysates were then analyzed for cleaved caspase 3 by western blotting. Data are from one experiment, representative of two. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/21810214), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Human Human/Mouse Cleaved Caspase-3 (Asp175) Antibody by Immunohistochemistry View Larger

Detection of Human Human/Mouse Cleaved Caspase-3 (Asp175) Antibody by Immunohistochemistry Assessment of the skin structure and apoptosis upon treatment with wound gels. Representative images and zoom-ins of the boxed areas of haematoxylin and eosin (H&E)-stained tape-stripped (TS) sections from biopsies (age 38 years) either left untreated or treated with indicated wound gels using a light microscope. The presence of caspase-3 expressing cells was evaluated using a caspase-3 (CASP3) antibody and visualisation with a secondary Alexa Fluor™ 546 AB (red). Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/36261541), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Reconstitute at 0.5 mg/mL in sterile PBS.
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Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
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Background: Caspase-3

Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.

Entrez Gene IDs
836 (Human); 12367 (Mouse)
Alternate Names
Apopain; apoptosis-related cysteine protease; CASP3; CASP-3; caspase 3, apoptosis-related cysteine peptidase; Caspase3; Caspase-3; CPP32; CPP-32; CPP32B; CPP32SREBP cleavage activity 1; Cysteine protease CPP32; EC 3.4.22; EC 3.4.22.56; LICE-1; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; YAMA

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Citations for Human/Mouse Cleaved Caspase-3 (Asp175) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

99 Citations: Showing 1 - 10
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  1. ER Redox Homeostasis Regulates Proinsulin Trafficking and Insulin Granule Formation in the Pancreatic Islet beta-Cell
    Authors: Rohli KE, Boyer CK, Bearrows SC et al.
    Function (Oxford, England)
  2. Impaired mitochondrial fatty acid synthesis leads to neurodegeneration in mice.
    Authors: Nair RR, koivisto H, Jokivarsi k et al.
    J. Neurosci.
  3. Downregulation of CDC20 Increases Radiosensitivity through Mcl-1/p-Chk1-Mediated DNA Damage and Apoptosis in Tumor Cells
    Authors: Yang Gao, Pengbo Wen, Bin Chen, Guanshuo Hu, Lijun Wu, An Xu et al.
    International Journal of Molecular Sciences
  4. Unconventional Secretion of Adipocyte Fatty Acid Binding Protein 4 Is Mediated By Autophagic Proteins in a Sirtuin-1–Dependent Manner
    Authors: Ajeetha Josephrajan, Ann V. Hertzel, Ellie K. Bohm, Michael W. McBurney, Shin-Ichiro Imai, Douglas G. Mashek et al.
    Diabetes
  5. DL-3-n-butylphthalide protects endothelial cells against advanced glycation end product-induced injury by attenuating oxidative stress and inflammation responses
    Authors: Chang-Yun Liu, Zhen-Hua Zhao, Zhi-Ting Chen, Chun-Hui Che, Zhang-Yu Zou, Xiao-Min Wu et al.
    Experimental and Therapeutic Medicine
  6. Augmin deficiency in neural stem cells causes p53-dependent apoptosis and aborts brain development
    Authors: Ricardo Viais, Marcos Fariña-Mosquera, Marina Villamor-Payà, Sadanori Watanabe, Lluís Palenzuela, Cristina Lacasa et al.
    eLife
  7. Knockdown of anti-silencing function 1B histone chaperone induces cell apoptosis via repressing PI3K/Akt pathway in prostate cancer
    Authors: Guangye Han, Xinjun Zhang, Pei Liu, Quanfeng Yu, Zeyu Li, Qinnan Yu et al.
    International Journal of Oncology
  8. Knockdown of RRM1 in tumor cells promotes radio-/chemotherapy induced ferroptosis by regulating p53 ubiquitination and p21-GPX4 signaling axis
    Authors: Yang Gao, Bin Chen, Ruru Wang, An Xu, Lijun Wu, Huayi Lu et al.
    Cell Death Discovery
  9. Endoplasmic reticulum stress and IRE-1 signaling cause apoptosis in colon cancer cells in response to andrographolide treatment
    Authors: Aditi Banerjee, Hafiz Ahmed, Peixin Yang, Steven J. Czinn, Thomas G. Blanchard
    Oncotarget
  10. Intercellular Adhesion Molecule-1-Induced Posttraumatic Brain Injury Neuropathology in the Prefrontal Cortex and Hippocampus Leads to Sensorimotor Function Deficits and Psychological Stress
    Authors: Bhowmick S, Malat A, Caruso D Et Al.
    eNeuro
  11. Impact of Andrographolide and Melatonin Combinatorial Drug Therapy on Metastatic Colon Cancer Cells and Organoids
    Authors: Neha Sharda, Tamaki Ikuse, Elizabeth Hill, Sonia Garcia, Steven J Czinn, Andrea Bafford et al.
    Clin Med Insights Oncol
  12. Smac-mimetic enhances antitumor effect of standard chemotherapy in ovarian cancer models via Caspase 8-independent mechanism
    Authors: Lidia F. Hernandez, Angie B. Dull, Soumya Korrapati, Christina M. Annunziata
    Cell Death Discovery
  13. Preclinical evaluation of the first intravenous small molecule MDM2 antagonist alone and in combination with temozolomide in neuroblastoma
    Authors: Lindi Chen, Fabio Pastorino, Philip Berry, Jennifer Bonner, Calum Kirk, Katrina M. Wood et al.
    International Journal of Cancer
  14. Dysfunctional SEMA3E signaling underlies gonadotropin-releasing hormone neuron deficiency in Kallmann syndrome
    Authors: Anna Cariboni, Valentina André, Sophie Chauvet, Daniele Cassatella, Kathryn Davidson, Alessia Caramello et al.
    Journal of Clinical Investigation
  15. High-Mobility Group Box-1 Induces Decreased Brain-Derived Neurotrophic Factor-Mediated Neuroprotection in the Diabetic Retina
    Authors: Ahmed M. Abu El-Asrar, Mohd Imtiaz Nawaz, Mohammad Mairaj Siddiquei, Abdullah S. Al-Kharashi, Dustan Kangave, Ghulam Mohammad
    Mediators of Inflammation
  16. Effect of brefeldin A and castanospermine on resistant cell lines as supplements in anticancer therapy
    Authors: KAROLINA WOJTOWICZ, RADOSŁAW JANUCHOWSKI, PATRYCJA SOSIŃSKA, MICHAŁ NOWICKI, MACIEJ ZABEL
    Oncology Reports
  17. Epidermal Rac1 regulates the DNA damage response and protects from UV-light-induced keratinocyte apoptosis and skin carcinogenesis
    Authors: J Deshmukh, R Pofahl, I Haase
    Cell Death Dis, 2017-03-09;8(3):e2664.
  18. Intratumoral injection of pEGFC1-IGFBP7 inhibits malignant melanoma growth in C57BL/6J mice by inducing apoptosis and down-regulating VEGF expression
    Authors: Rong-Yi Chen, Hong-Xiang Chen, Ping Jian, Li Xu, Juan Li, Yi-Ming Fan et al.
    Oncology Reports
  19. Development of a quantitative pharmacodynamic assay for apoptosis in fixed tumor tissue and its application in distinguishing cytotoxic drug—induced DNA double strand breaks from DNA double strand breaks associated with apoptosis
    Authors: Angie B. Dull, Deborah Wilsker, Melinda Hollingshead, Christina Mazcko, Christina M. Annunziata, Amy K. LeBlanc et al.
    Oncotarget
  20. A piRNA-like Small RNA Induces Chemoresistance to Cisplatin-Based Therapy by Inhibiting Apoptosis in Lung Squamous Cell Carcinoma
    Authors: Yuyan Wang, Tyler Gable, Mark Z. Ma, David Clark, Jun Zhao, Yi Zhang et al.
    Molecular Therapy - Nucleic Acids
  21. Notch1 is required to maintain supporting cell identity and vestibular function during maturation of the mammalian balance organs
    Authors: Heffer, A;Lee, C;Holt, JC;Kiernan, AE;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  22. Interferon-? Overexpression in Adipose Tissue-Derived Stem Cells Induces HepG2 and Macrophage Cell Death in Liver Tumor Organoids via Induction of TNF-Related Apoptosis-Inducing Ligand Expression
    Authors: Yoon, Y;Kim, CW;Kim, MY;Baik, SK;Jung, PY;Eom, YW;
    International journal of molecular sciences
    Species: Human
    Sample Types: Organoids
    Applications: Western Blot, IHC
  23. Improving anti-tumor efficacy of low-dose Vincristine in rhabdomyosarcoma via the combination therapy with FOXM1 inhibitor RCM1
    Authors: Donovan J, Deng Z, Bian F et al.
    Frontiers in oncology
  24. CCR3 blockage elicits polyploidization associated with the signatures of epithelial-mesenchymal transition in carcinoma cell lines
    Authors: Y Kaibori, D Nagakubo
    Cancer Gene Therapy, 2022-09-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  25. Cellular recovery after prolonged warm ischaemia of the whole body
    Authors: D Andrijevic, Z Vrselja, T Lysyy, S Zhang, M Skarica, A Spajic, D Dellal, SL Thorn, RB Duckrow, S Ma, PQ Duy, AU Isiktas, D Liang, M Li, SK Kim, SG Daniele, K Banu, S Perincheri, MC Menon, A Huttner, KN Sheth, KT Gobeske, GT Tietjen, HP Zaveri, SR Latham, AJ Sinusas, N Sestan
    Nature, 2022-08-03;608(7922):405-412.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  26. Insights into the Pharmacokinetics and In Vitro Cell-Based Studies of the Imidazoline I2 Receptor Ligand B06
    Authors: A Bagán, JA Morales-Ga, C Griñán-Fer, C Díaz, J Pérez Del, MC Ramos, F Vicente, B Pérez, J Brea, MI Loza, M Pallàs, C Escolano
    International Journal of Molecular Sciences, 2022-05-12;23(10):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. KDM5B promotes immune evasion by recruiting SETDB1 to silence retroelements
    Authors: SM Zhang, WL Cai, X Liu, D Thakral, J Luo, LH Chan, MK McGeary, E Song, KRM Blenman, G Micevic, S Jessel, Y Zhang, M Yin, CJ Booth, LB Jilaveanu, W Damsky, M Sznol, HM Kluger, A Iwasaki, MW Bosenberg, Q Yan
    Nature, 2021-10-20;598(7882):682-687.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  28. Measles virus exits human airway epithelia within dislodged metabolically active infectious centers
    Authors: CE Hippee, BK Singh, AL Thurman, AL Cooney, AA Pezzulo, R Cattaneo, PL Sinn
    PloS Pathogens, 2021-08-12;17(8):e1009458.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  29. CD8+ tissue-resident memory T cells promote liver fibrosis resolution by inducing apoptosis of hepatic stellate cells
    Authors: Y Koda, T Teratani, PS Chu, Y Hagihara, Y Mikami, Y Harada, H Tsujikawa, K Miyamoto, T Suzuki, N Taniki, T Sujino, M Sakamoto, T Kanai, N Nakamoto
    Nature Communications, 2021-07-22;12(1):4474.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. NF1 mutation drives neuronal�activity-dependent initiation of optic glioma
    Authors: Y Pan, JD Hysinger, T Barron, NF Schindler, O Cobb, X Guo, B Yalç?n, C Anastasaki, SB Mulinyawe, A Ponnuswami, S Scheaffer, Y Ma, KC Chang, X Xia, JA Toonen, JJ Lennon, EM Gibson, JR Huguenard, LM Liau, JL Goldberg, M Monje, DH Gutmann
    Nature, 2021-05-26;594(7862):277-282.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  31. Spaceflight and hind limb unloading induces an arthritic phenotype in knee articular cartilage and menisci of rodents
    Authors: AT Kwok, NS Mohamed, JF Plate, RR Yammani, S Rosas, TA Bateman, E Livingston, JE Moore, BA Kerr, J Lee, CM Furdui, L Tan, ML Bouxsein, VL Ferguson, LS Stodieck, DC Zawieja, MD Delp, XW Mao, JS Willey
    Scientific Reports, 2021-05-18;11(1):10469.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  32. Ziyuglycoside II inhibits the growth of digestive system cancer cells through multiple mechanisms
    Authors: Y Zhong, XY Li, F Zhou, YJ Cai, R Sun, RP Liu
    Chinese journal of natural medicines, 2021-05-01;19(5):351-363.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  33. Facilitative lysosomal transport of bile acids alleviates ER stress in mouse hematopoietic precursors
    Authors: AK Persaud, S Nair, MF Rahman, R Raj, B Weadick, D Nayak, C McElroy, M Shanmugam, S Knoblaugh, X Cheng, R Govindaraj
    Nature Communications, 2021-02-23;12(1):1248.
    Species: Mouse
    Sample Types:
    Applications: ICC/IF
  34. Genome-wide analysis of copy number alterations led to the characterisation of PDCD10 as oncogene in ovarian cancer
    Authors: C De Marco, P Zoppoli, N Rinaldo, S Morganella, M Morello, V Zuccalà, MV Carriero, D Malanga, R Chirillo, P Bruni, C Malzoni, D Di Vizio, R Venturella, F Zullo, A Rizzuto, M Ceccarelli, G Ciliberto, G Viglietto
    Translational Oncology, 2021-01-27;14(3):101013.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  35. MicroRNA miR-24-3p reduces DNA damage responses, apoptosis, and susceptibility to chronic obstructive pulmonary disease
    Authors: J Nouws, F Wan, E Finnemore, W Roque, SJ Kim, IS Bazan, CX Li, CM Sköld, Q Dai, X Yan, M Chioccioli, V Neumeister, CJ Britto, J Sweasy, RS Bindra, ÅM Wheelock, JL Gomez, N Kaminski, PJ Lee, M Sauler
    JCI Insight, 2021-01-25;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  36. The interruption of atypical PKC signaling and Temozolomide combination therapy against glioblastoma
    Authors: A Dey, SMA Islam, R Patel, M Acevedo-Du
    Cell Signal, 2020-11-02;77(0):109819.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  37. Transcription factor 21 expression in injured podocytes of glomerular diseases
    Authors: J Usui, M Yaguchi, S Yamazaki, M Takahashi-, T Kawamura, S Kaneko, SV Seshan, P Ronco, K Yamagata
    Sci Rep, 2020-07-13;10(1):11516.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  38. Calebin A Potentiates the Effect of 5-FU and TNF-&beta (Lymphotoxin &alpha) against Human Colorectal Cancer Cells: Potential Role of NF-&kappaB
    Authors: C Buhrmann, AB Kunnumakka, B Popper, M Majeed, BB Aggarwal, M Shakibaei
    Int J Mol Sci, 2020-03-31;21(7):.
    Species: Human
    Sample Types: Whole Cell
    Applications: IF
  39. Effects of Pseudomonas aeruginosa on Microglial-Derived Extracellular Vesicle Biogenesis and Composition
    Authors: LB Jones, S Kumar, CR Bell, VA Peoples, BJ Crenshaw, MT Coats, JA Scoffield, GC Rowe, B Sims, QL Matthews
    Pathogens, 2019-12-14;8(4):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Dot Blot
  40. Piwi-Interacting RNA1037 Enhances Chemoresistance and Motility in Human Oral Squamous Cell Carcinoma Cells
    Authors: G Li, X Wang, C Li, S Hu, Z Niu, Q Sun, M Sun
    Onco Targets Ther, 2019-12-04;12(0):10615-10627.
    Species: Xenograft
    Sample Types: Cell Lysates
    Applications: Western Blot
  41. Tissue-regenerative potential of the secretome of gamma-irradiated peripheral blood mononuclear cells is mediated via TNFRSF1B-induced necroptosis
    Authors: E Simader, L Beer, M Laggner, V Vorstandle, A Gugerell, M Erb, P Kalinina, D Copic, D Moser, A Spittler, E Tschachler, H Jan Ankers, M Mildner
    Cell Death Dis, 2019-09-30;10(10):729.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  42. Influence of Hypoxic Preservation Temperature on Endothelial Cells and Kidney Integrity
    Authors: S Giraud, C Steichen, P Couturier, S Tillet, V Mallet, R Coudroy, JM Goujon, P Hannaert, T Hauet
    Biomed Res Int, 2019-06-04;2019(0):8572138.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC-P
  43. Elaeagnus glabra f. oxyphylla Attenuates Scopolamine-Induced Learning and Memory Impairments in Mice by Improving Cholinergic Transmission via Activation of CREB/NGF Signaling
    Authors: E Sohn, HS Lim, YJ Kim, BY Kim, JH Kim, SJ Jeong
    Nutrients, 2019-05-28;11(6):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  44. The effect of the cholinergic anti-inflammatory pathway on collagen-induced arthritis involves the modulation of dendritic cell differentiation
    Authors: D Liu, T Li, H Luo, X Zuo, S Liu, S Wu
    Arthritis Res. Ther., 2018-11-28;20(1):263.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  45. Lect2 deficiency is characterised by altered cytokine levels and promotion of intestinal tumourigenesis
    Authors: KR Greenow, M Zverev, S May, H Kendrick, GT Williams, T Phesse, L Parry
    Oncotarget, 2018-11-23;9(92):36430-36443.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  46. Progranulin protects the mouse retina under hypoxic conditions via inhibition of the Toll?like receptor?4?NADPH oxidase 4 signaling pathway
    Authors: ZP You, MJ Yu, YL Zhang, K Shi
    Mol Med Rep, 2018-11-08;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  47. The protective effect of rifampicin on behavioral deficits, biochemical, and neuropathological changes in a cuprizone model of demyelination
    Authors: H Zahednasab, M Firouzi, S Kaboudania, Z Mojallal-T, S Karampour, H Keyvani
    Cytokine, 2018-10-24;113(0):417-426.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  48. Expression of the neuroprotective protein aryl hydrocarbon receptor nuclear translocator 2 correlates with neuronal stress and disability in models of multiple sclerosis
    Authors: T Rahim, P Becquart, ME Baeva, J Quandt
    J Neuroinflammation, 2018-09-19;15(1):270.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  49. Bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function
    Authors: R Hamon, HB Tran, E Roscioli, M Ween, H Jersmann, S Hodge
    Sci Rep, 2018-09-07;8(1):13424.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  50. The protective effects of total paeony glycoside on ischemia/reperfusion injury in H9C2 cells via inhibition of the PI3K/Akt signaling pathway
    Authors: P Shen, J Chen, M Pan
    Mol Med Rep, 2018-07-30;18(3):3332-3340.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  51. Effect of the S-nitrosoglutathione reductase inhibitor N6022 on bronchial hyperreactivity in asthma
    Authors: LG Que, Z Yang, NL Lugogo, RK Katial, SA Shoemaker, JM Troha, DM Rodman, RM Tighe, M Kraft
    Immun Inflamm Dis, 2018-04-11;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  52. Trabecular meshwork morphogenesis: A comparative analysis of wildtype and anterior segment dysgenesis mouse models
    Authors: RL Rausch, RT Libby, AE Kiernan
    Exp. Eye Res., 2018-02-13;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  53. Kruppel-like factor 4-dependent Staufen1-mediated mRNA decay regulates cortical neurogenesis
    Authors: BS Moon, J Bai, M Cai, C Liu, J Shi, W Lu
    Nat Commun, 2018-01-26;9(1):401.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  54. Caspases are key regulators of inflammatory and innate immune responses mediated by TLR3 in vivo
    Authors: A Shaalan, G Carpenter, G Proctor
    Mol. Immunol., 2018-01-11;94(0):190-199.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  55. Loss of Tmem30a leads to photoreceptor degeneration
    Authors: L Zhang, Y Yang, S Li, S Zhang, X Zhu, Z Tai, M Yang, Y Liu, X Guo, B Chen, Z Jiang, F Lu, X Zhu
    Sci Rep, 2017-08-24;7(1):9296.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  56. The protective effects of Resveratrol against radiation-induced intestinal injury
    Authors: H Zhang, H Yan, X Zhou, H Wang, Y Yang, J Zhang, H Wang
    BMC Complement Altern Med, 2017-08-16;17(1):410.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  57. Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration
    Authors: F Sallustio, C Curci, A Aloisi, CC Toma, E Marulli, G Serino, SN Cox, G De Palma, A Stasi, C Divella, R Rinaldi, FP Schena
    Sci Rep, 2017-08-15;7(1):8225.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  58. WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response
    Authors: S Janczar, J Nautiyal, Y Xiao, E Curry, M Sun, E Zanini, AJ Paige, H Gabra
    Cell Death Dis, 2017-07-27;8(7):e2955.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  59. Potent Vasoconstrictor Kisspeptin-10 Induces Atherosclerotic Plaque Progression and Instability: Reversal by its Receptor GPR54 Antagonist
    Authors: K Sato, R Shirai, M Hontani, R Shinooka, A Hasegawa, T Kichise, T Yamashita, H Yoshizawa, R Watanabe, TA Matsuyama, H Ishibashi-, S Koba, Y Kobayashi, T Hirano, T Watanabe
    J Am Heart Assoc, 2017-04-14;6(4):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  60. Ionizing radiation regulates long non-coding RNAs in human peripheral blood mononuclear cells
    Authors: Lucian Beer
    J. Radiat. Res, 2017-03-01;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  61. Resveratrol fuels HER2 and ER?-positive breast cancer behaving as proteasome inhibitor
    Authors: C Andreani, C Bartolacci, K Wijnant, R Crinelli, M Bianchi, M Magnani, A Hysi, M Iezzi, A Amici, C Marchini
    Aging (Albany NY), 2017-02-26;9(2):508-523.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  62. Formyl peptide receptor 1 suppresses gastric cancer angiogenesis and growth by exploiting inflammation resolution pathways
    Authors: N Prevete, F Liotti, A Illiano, A Amoresano, P Pucci, A de Paulis, RM Melillo
    Oncoimmunology, 2017-02-21;6(4):e1293213.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  63. Melatonin alleviates lipopolysaccharide-compromised integrity of blood-brain barrier through activating AMP-activated protein kinase in old mice
    Authors: X Wang, GX Xue, WC Liu, H Shu, M Wang, Y Sun, X Liu, YE Sun, CF Liu, J Liu, W Liu, X Jin
    Aging Cell, 2017-02-03;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  64. H-Ras and K-Ras oncoproteins induce different tumor spectra when driven by the same regulatory sequences
    Cancer Res., 2016-11-21;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  65. Impaired Epidermal to Dendritic T Cell Signaling Slows Wound Repair in Aged Skin
    Authors: Elaine Fuchs
    Cell, 2016-11-17;167(5):1323-1338.e14.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  66. Chimeric Mice with Competent Hematopoietic Immunity Reproduce Key Features of Severe Lassa Fever
    PLoS Pathog, 2016-05-18;12(5):e1005656.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  67. Antitumor cytotoxicity induced by bone-marrow-derived antigen-presenting cells is facilitated by the tumor suppressor protein p53 via regulation of IL-12
    Authors: Tania L Slatter
    Oncoimmunology, 2015-12-17;5(3):e1112941.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  68. Latent herpes simplex virus 1 infection does not induce apoptosis in human trigeminal Ganglia.
    Authors: Himmelein S, Lindemann A, Sinicina I, Strupp M, Brandt T, Hufner K
    J Virol, 2015-03-11;89(10):5747-50.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  69. The formyl peptide receptor 1 exerts a tumor suppressor function in human gastric cancer by inhibiting angiogenesis.
    Authors: Prevete N, Liotti F, Visciano C, Marone G, Melillo R, de Paulis A
    Oncogene, 2014-09-29;34(29):3826-38.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  70. The deubiquitinase USP28 controls intestinal homeostasis and promotes colorectal cancer.
    Authors: Diefenbacher M, Popov N, Blake S, Schulein-Volk C, Nye E, Spencer-Dene B, Jaenicke L, Eilers M, Behrens A
    J Clin Invest, 2014-06-24;124(8):3407-18.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  71. Edaravone protect against retinal damage in streptozotocin-induced diabetic mice.
    Authors: Yuan, Dongqing, Xu, Yidan, Hang, Hui, Liu, Xiaoyi, Chen, Xi, Xie, Ping, Yuan, Songtao, Zhang, Weiwei, Lin, Xiaojun, Liu, Qinghuai
    PLoS ONE, 2014-06-04;9(6):e99219.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  72. Triptolide reverses hypoxia-induced epithelial-mesenchymal transition and stem-like features in pancreatic cancer by NF-kappaB downregulation.
    Authors: Liu L, Salnikov A, Bauer N, Aleksandrowicz E, Labsch S, Nwaeburu C, Mattern J, Gladkich J, Schemmer P, Werner J, Herr I
    Int J Cancer, 2014-05-15;134(10):2489-503.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  73. Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-beta.
    Authors: Wiener Z, Band A, Kallio P, Hogstrom J, Hyvonen V, Kaijalainen S, Ritvos O, Haglund C, Kruuna O, Robine S, Louvard D, Ben-Neriah Y, Alitalo K
    Proc Natl Acad Sci U S A, 2014-05-13;111(21):E2229-36.
    Species: Human, Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  74. Evaluation of antiviral efficacy of ribavirin, arbidol, and T-705 (favipiravir) in a mouse model for Crimean-Congo hemorrhagic fever.
    Authors: Oestereich L, Rieger T, Neumann M, Bernreuther C, Lehmann M, Krasemann S, Wurr S, Emmerich P, de Lamballerie X, Olschlager S, Gunther S
    PLoS Negl Trop Dis, 2014-05-01;8(5):e2804.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  75. Proinflammatory cytokines differentially regulate adipocyte mitochondrial metabolism, oxidative stress, and dynamics.
    Authors: Hahn W, Kuzmicic J, Burrill J, Donoghue M, Foncea R, Jensen M, Lavandero S, Arriaga E, Bernlohr D
    Am J Physiol Endocrinol Metab, 2014-03-04;306(9):E1033-45.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  76. The novel c-Met inhibitor cabozantinib overcomes gemcitabine resistance and stem cell signaling in pancreatic cancer.
    Authors: Hage C, Rausch V, Giese N, Giese T, Schonsiegel F, Labsch S, Nwaeburu C, Mattern J, Gladkich J, Herr I
    Cell Death Dis, 2013-05-09;4(0):e627.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  77. Lipopeptide biosurfactant pseudofactin II induced apoptosis of melanoma A 375 cells by specific interaction with the plasma membrane.
    Authors: Janek T, Krasowska A, Radwanska A, Lukaszewicz M
    PLoS ONE, 2013-03-06;8(3):e57991.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  78. The ROS scavenger, NAC, regulates hepatic Vα14iNKT cells signaling during Fas mAb-dependent fulminant liver failure.
    Authors: Downs I, Liu J, Aw TY, Adegboyega PA, Ajuebor MN
    PLoS ONE, 2012-06-06;7(6):e38051.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  79. FRMD4A upregulation in human squamous cell carcinoma promotes tumor growth and metastasis and is associated with poor prognosis.
    Authors: Goldie S, Mulder K, Tan D, Lyons S, Sims A, Watt F
    Cancer Res, 2012-05-07;72(13):3424-36.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  80. Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt.
    Authors: Deng J, Han Y, Yan C, Tian X, Tao J, Kang J, Li S
    Apoptosis, 2010-04-01;15(4):463-73.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  81. In-vivo transfection of pcDNA3.1-IGFBP7 inhibits melanoma growth in mice through apoptosis induction and VEGF downexpression.
    Authors: Chen RY, Chen HX, Lin JX
    J. Exp. Clin. Cancer Res., 2010-02-16;29(0):13.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  82. Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation.
    Authors: Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, Kang J, Zhang X, Yan C
    J. Mol. Cell. Cardiol., 2010-01-06;48(6):1225-35.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  83. Neuropsin promotes oligodendrocyte death, demyelination and axonal degeneration after spinal cord injury.
    Authors: Terayama R, Bando Y, Murakami K, Kato K, Kishibe M, Yoshida S
    Neuroscience, 2007-07-12;148(1):175-87.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  84. Chondrocyte hypertrophy and apoptosis induced by GROalpha require three-dimensional interaction with the extracellular matrix and a co-receptor role of chondroitin sulfate and are associated with the mitochondrial splicing variant of cathepsin B.
    Authors: Olivotto E, Vitellozzi R, Fernandez P, Falcieri E, Battistelli M, Burattini S, Flamigni F, Santi S, Borzi RM
    J. Cell. Physiol., 2007-02-01;210(2):417-27.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  85. Changes in lamina structure are followed by spatial reorganization of heterochromatic regions in caspase-8-activated human mesenchymal stem cells.
    Authors: Raz V, Carlotti F, Vermolen BJ, van der Poel E, Sloos WC, Knaan-Shanzer S, de Vries AA, Hoeben RC, Young IT, Tanke HJ, Garini Y, Dirks RW
    J. Cell. Sci., 2006-09-26;119(0):4247-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  86. Pathological Significance and Prognostic Roles of Thrombospondin-3, 4 and 5 in Bladder Cancer
    Authors: JUNKI HARADA, YASUYOSHI MIYATA, KYOHEI ARAKI, TSUYOSHI MATSUDA, YOSHIAKI NAGASHIMA, YUTA MUKAE et al.
    In Vivo
  87. Improving anti-tumor efficacy of low-dose Vincristine in rhabdomyosarcoma via the combination therapy with FOXM1 inhibitor RCM1
    Authors: Donovan J, Deng Z, Bian F et al.
    Frontiers in oncology
  88. Pharmacodynamic markers and clinical results from the phase 2 study of the SMAC mimetic birinapant in women with relapsed platinum-resistant or -refractory epithelial ovarian cancer
    Authors: Anne M. Noonan, Kristen P. Bunch, Jin-Qiu Chen, Michelle A. Herrmann, Jung-Min Lee, Elise C. Kohn et al.
    Cancer
  89. Stromal expression of Fer suppresses tumor progression in renal cell carcinoma and is a predictor of survival
    Authors: Kensuke Mitsunari, Yasuyoshi Miyata, Shin-Ichi Watanabe, Akihiro Asai, Takuji Yasuda, Shigeru Kanda et al.
    Oncology Letters
  90. Exosomal miRNA Let-7 from Menstrual Blood-Derived Endometrial Stem Cells Alleviates Pulmonary Fibrosis through Regulating Mitochondrial DNA Damage
    Authors: Sun L, Zhu M, Feng W, et al.
    Oxidative Medicine and Cellular Longevity
  91. Accumulation of Nucleolar Inorganic Polyphosphate Is a Cellular Response to Cisplatin-Induced Apoptosis
    Authors: Lihan Xie, Asavari Rajpurkar, Ellen Quarles, Nicole Taube, Akash S. Rai, Jake Erba et al.
    Frontiers in Oncology
  92. Protein kinase CK2 inhibition suppresses neointima formation via a proline-rich homeodomain-dependent mechanism
    Authors: K.S. Wadey, B.A. Brown, G.B. Sala-Newby, P.-S. Jayaraman, K. Gaston, S.J. George
    Vascular Pharmacology
  93. Poly (ADP-Ribose) Polymerase Mediates Diabetes-Induced Retinal Neuropathy
    Authors: Ghulam Mohammad, Mohammad Mairaj Siddiquei, Ahmed M. Abu El-Asrar
    Mediators of Inflammation
  94. FOXF1 transcription factor promotes lung morphogenesis by inducing cellular proliferation in fetal lung mesenchyme
    Authors: Vladimir Ustiyan, Craig Bolte, Yufang Zhang, Lu Han, Yan Xu, Katherine E. Yutzey et al.
    Developmental Biology
  95. Mitochondrial serine protease Omi/HtrA2 accentuates brain ischemia/reperfusion injury in rats and oxidative stress injury in vitro by modulating mitochondrial stress proteins CHOP and ClpP and physically interacting with mitochondrial fusion protein OPA1
    Authors: Hailong You, Yao Jin, Jinsong Kang, Ying Mao, Jing Su, Liankun Sun et al.
    Bioengineered
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    Authors: X Wang, W Liu, D Zhuang, S Hong, J Chen
    Oncotarget, 2017-10-04;8(52):90132-90143.
  97. Neurodegeneration and Sensorimotor Deficits in the Mouse Model of Traumatic Brain Injury
    Authors: Saurav Bhowmick, Veera D‘Mello, Nizmi Ponery, P. M. Abdul-Muneer
    Brain Sciences
  98. Pro-inflammatory role of Wnt/ beta -catenin signaling in endothelial dysfunction
    Authors: Kerry S. Wadey, Alexandros Somos, Genevieve Leyden, Hazel Blythe, Jeremy Chan, Lawrence Hutchinson et al.
    Frontiers in Cardiovascular Medicine
  99. TLR9-induced interferon beta is associated with protection from gammaherpesvirus-induced exacerbation of lung fibrosis.
    Authors: Luckhardt Tracy R, Coomes Stephanie M, Trujillo Glenda et al.
    Fibrogenesis Tissue Repair.

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Reviews for Human/Mouse Cleaved Caspase-3 (Asp175) Antibody

Average Rating: 4.6 (Based on 8 Reviews)

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Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
By Anonymous on 09/23/2023
Application: WB Sample Tested: Breast cancer cells Species: Human

In the immunoblot, our goal is to see the expressing differences within different treatments, and we clearly saw the bands. Although there were some non-specific bands, but they did not affect the discrimination between groups.

Western Blot Human/Mouse Cleaved Caspase-3 (Asp175) Antibody MAB835
Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
By Anonymous on 09/22/2022
Application: B/N Sample Tested: HEK293 human embryonic kidney cell line Species: Human

I used it to block this protein.

Block/Neutralize Human/Mouse Cleaved Caspase-3 (Asp175) Antibody MAB835
Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
By Anonymous on 08/04/2021
Application: IHC Sample Tested: Kidney tissue Species: Mouse
Immunohistochemistry Human/Mouse Cleaved Caspase-3 (Asp175) Antibody MAB835
Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
By Anonymous on 02/16/2020
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human
Western Blot Human/Mouse Cleaved Caspase-3 (Asp175) Antibody MAB835
Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
By Anonymous on 01/17/2018
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human
Western Blot Human/Mouse Cleaved Caspase-3 (Asp175) Antibody MAB835
Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
By Anonymous on 09/27/2017
Application: Flow Sample Tested: Liver tissue Species: Mouse
Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
By Byoung San Moon on 09/07/2017
Application: IHC Sample Tested: Embryonic brain Species: Mouse
Immunohistochemistry Human/Mouse Cleaved Caspase-3 (Asp175) Antibody MAB835
Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
By Anonymous on 02/08/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: IPS2 induced pluripotent stem cells derived cells Species: Human
Immunocytochemistry/Immunofluorescence Human/Mouse Cleaved Caspase-3 (Asp175) Antibody MAB835