Human/Mouse DAPP1 Antibody

Catalog #: MAB7024 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7024
MAB7024-SP

Detection of Human and Mouse DAPP1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse DAPP1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human DAPP1 in direct ELISAs. Detects human and mouse DAPP1 in Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 716804

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DAPP1
Gly2-Ser163
Accession # Q9UN19

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse DAPP1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse DAPP1 Monoclonal Antibody (Catalog # MAB7024) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DAPP1 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DAPP1

DAPP1, also known as Bam32, is a 32 kDa intracellular adaptor protein for tyrosine-phosphorylated proteins and 3-phosphoinositides. It is expressed in B cells, T cells, and mast cells and plays a role in signal transduction during the activation of these cell types. DAPP1 is also required for progression of germinal center reactions and the adhesion of B cells to T cells. It is recruited to the plasma membrane following PI 3-kinase activation. DAPP1 contains one SH2 (aa 34-129) and one PH (aa 164-259) domain. Within aa 2-163, human DAPP1 shares approximately 90% aa sequence identity with mouse and rat DAPP1. Alternate splicing generates an additional isoform with a 22 aa substitution at the C-terminus.

Long Name

Dual Adaptor of Phosphotyrosine and 3-Phosphoinositides 1

Entrez Gene IDs

27071 (Human); 26377 (Mouse); 362046 (Rat)

Alternate Names

B lymphocyte adapter protein Bam32; BAM32; BAM32DKFZp667E0716; B-cell adapter molecule of 32 kDa; DAPP1; dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide; dual adaptor of phosphotyrosine and 3-phosphoinositides; hDAPP1