Human/Mouse DDX5 Antibody

Catalog #: MAB6370
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6370
MAB6370-SP

Detection of Human and Mouse DDX5 by Western Blot.

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All DDX5 Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse DDX5 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse DDX5 in Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 632813

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DDX5
Asn448-Gln614
Accession # P17844

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse DDX5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse DDX5 Monoclonal Antibody (Catalog # MAB6370) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DDX5 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DDX5

DDX5, also known as p68 RNA helicase, is a 614 amino acid (aa), 68 kDa member of the DEAD box family of RNA helicases that contain the conserved Asp‑Glu‑Ala‑Asp (DEAD) motif. DDX5 influences RNA transport, transcription, ribosome assembly, spliceosome function, RNA degradation, and the initiation of translation. DDX5 shares 87% aa identity with DDX17 within the central region and the two are often expressed together. The C-terminal region used as an immunogen shares 96% and 99% aa identity with mouse and rat DDX5, respectively, but lies mainly outside the DDX17 homology region.

Long Name

DEAD Box Protein 5

Entrez Gene IDs

1655 (Human); 13207 (Mouse); 287765 (Rat)

Alternate Names

B-cell CLL/lymphoma 7 protein family member C; B-cell CLL/lymphoma 7C; DDX5; DEAD/H; G17P1; HELR; HLR1; HUMP68

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse DDX5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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