Human/Mouse DGK-iota Antibody

Catalog #: MAB6435
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6435
MAB6435-SP

Detection of Human and Mouse DGK‑ iota by Western Blot.

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All DGK-iota Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse DGK-iota Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse DGK‑ iota in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human DGK-alpha,
-beta, -δ, -epsilon, -eta, -gamma, -kappa, -theta, or -zeta is observed.

Source

Monoclonal Mouse IgG_2A Clone # 648538

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DGK‑ iota
Ser925-Val1065 (predicted)
Accession # O75912

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse DGK‑ iota by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and mouse brain tissue. PVDF Membrane was probed with 1 µg/mL of Human/Mouse/Rat DGK-iota Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB6435) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK-iota at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DGK-iota

Diacylglycerol kinase iota (DGK-iota ) is an approximately 110 kDa kinase that phosphorylates the lipid diacylglycerol. DGK-iota also inhibits RasGRP3 and limits Rap1 activation. It contains two zinc finger regions (aa 178-232 and aa 251-310), a catalytic domain (aa 372-507), and two ankyrin repeats (aa 958-990 and aa 997-1026). Over aa 925-1065, human DGK-iota shares 94% and 96% aa sequence identity with mouse and rat DGK-iota, respectively.

Long Name

Diacylglycerol Kinase, iota 64kDa

Entrez Gene IDs

9162 (Human); 320127 (Mouse); 688705 (Rat)

Alternate Names

DAG kinase iota; DGKI; DGKiota; DGK-iota; diacylglycerol kinase iota; diacylglycerol kinase, iota; Diglyceride kinase iota; EC 2.7.1.107

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse DGK-iota Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Enhanced histamine-induced itch in diacylglycerol kinase iota knockout mice
Authors: VB Bartsch, JK Niehaus, B Taylor-Bla, MJ Zylka
PLoS ONE, 2019-06-05;14(6):e0217819.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC