Human/Mouse Doc2 alpha Antibody Summary
Met1-Lys114 (Gly48Ser)
Accession # Q14183
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse Doc2 alpha by Western Blot. Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, P19 mouse embryonal carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, SH-SY5Y human neuroblastoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Doc2a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7904) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Doc2a at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Doc2 alpha
DOC2A/Doc2 alpha (Double C2-like domain containing protein alpha) is a 44 kDa (predicted) monomeric member of the C2 domain-containing protein family of molecules. It is expressed in both neurons and mast cells, and appears to serve as an intracellular Ca++ sensor protein that regulates secretory vesicle release. In neurons, Doc2 alpha is normally bound to synaptic vesicles and interacts with Munc13-1 to promote secretory vesicle exocytosis through the cell membrane. In mast cells, a similar process occurs that involves Munc13-4 instead of Munc13-1. Human Doc2 alpha is 400 amino acids (aa) in length. It contains a Mid domain (aa 13-37) that binds Munc13-1, followed by one C2 domain that binds Ca++ and lipid (aa 91-195), and a second C2 domain that binds SNAP25 (253-356). There is one potential alternative start site 16 aa upstream of the standard site. Over aa 1-114, human Doc2 alpha shares 90% aa sequence identity with mouse Doc2 alpha. Human DOC2B is the product of a separate gene, and shares no meaningful aa sequence identity (< 30%) with human Doc2 alpha.
Product Datasheets
Citation for Human/Mouse Doc2 alpha Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Down-Regulation of Double C2 Domain Alpha Promotes the Formation of Hyperplastic Nerve Fibers in Aganglionic Segments of Hirschsprung's Disease
Authors: J Xiao, X Meng, K Chen, J Wang, L Wu, Y Chen, X Yu, J Feng, Z Li
International Journal of Molecular Sciences, 2022-09-06;23(18):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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