Home / FABP4/A-FABP / Human/Mouse FABP4/A-FABP Antibody

Human/Mouse FABP4/A-FABP Antibody

Catalog #: MAB3150
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3150
MAB3150-SP
Detection of Human and Mouse FABP4/A‑FABP by Western Blot.
2 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse FABP4/A-FABP Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human FABP4/A‑FABP in direct ELISAs and human and mouse FABP4/A-FABP in Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) FABP4, 50% cross-reactivity with recombinant human (rh) FABP3, 15% cross-reactivity with rhFABP3, and no cross-reactivity with rhFABP8 or rhFABP9 is observed.
Source
Monoclonal Rat IgG1 Clone # 804914
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FABP4/A‑FABP
Met1-Ala132
Accession # P15090
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3150).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human FABP4/A-FABP DuoSet ELISA Kit (Catalog # DY3150-05) for convenient development of a sandwich ELISA or the Human FABP4 Quantikine ELISA Kit (Catalog # DFBP40) for  a complete optimized ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse FABP4/A-FABP antibody by Western Blot. View Larger

Detection of Human and Mouse FABP4/A‑FABP by Western Blot. Western blot shows lysates of human adipose tissue and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human FABP4/A-FABP Monoclonal Antibody (Catalog # MAB3150) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FABP4/A-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ELISA View Larger

Human/Mouse FABP4/A‑FABP ELISA Standard Curve. Recombinant Human/Mouse FABP4/A‑FABP protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse FABP4/A‑FABP Monoclonal Antibody (Catalog # MAB3150) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human FABP4/A‑FABP Antigen Affinity-purified Polyclonal Antibody (AF3150) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP4/A-FABP

FABP4, also known as adipocyte P2 and A-FABP (adipocyte FABP), is a FABP family member that is expressed in adipocytes and monocyte-derived foam cells. It is a lipid transport protein that binds long chain fatty acid and retinoic acid. Human and mouse FABP4 share 91% amino acid sequence homology.

Long Name
Fatty Acid-Binding Protein 4
Entrez Gene IDs
2167 (Human); 11770 (Mouse); 79451 (Rat)
Alternate Names
Adipocyte lipid-binding protein; Adipocyte-type fatty acid-binding protein; AFABP; A-FABP; A-FABPAFABP; ALBP; aP2; FABP4; fatty acid binding protein 4, adipocyte; Fatty acid-binding protein 4; fatty acid-binding protein, adipocyte

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human/Mouse FABP4/A-FABP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Cathepsin B deficiency ameliorates liver lipid deposition, inflammatory cell infiltration, and fibrosis after diet-induced nonalcoholic steatohepatitis
    Authors: Fang W, Deng Z, Benadjaoud F et al.
    Transl Res
  2. Chronic DDE Exposure Modifies Mitochondrial Respiration during Differentiation of Human Adipose-Derived Mesenchymal Stem Cells into Mature Adipocytes
    Authors: I Kladnicka, M Cedikova, J Jedlicka, M Kohoutova, L Muller, I Plavinova, M Kripnerova, M Bludovska, J Kuncova, D Mullerova
    Biomolecules, 2021-07-21;11(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. A microRNA Cluster Controls Fat Cell Differentiation and Adipose Tissue Expansion By Regulating SNCG
    Authors: Ruth Rodríguez‐Barrueco, Jessica Latorre, Laura Devis‐Jáuregui, Aina Lluch, Nuria Bonifaci, Francisco J. Llobet et al.
    Advanced Science

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse FABP4/A-FABP Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse FABP4/A-FABP Antibody and earn rewards!

Have you used Human/Mouse FABP4/A-FABP Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review