Human/Mouse FoxJ3 Antibody

Catalog #: AF5786
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5786
AF5786-SP

Detection of Human FoxJ3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse FoxJ3 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse FoxJ3 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FoxJ3
Met375-Tyr552
Accession # NP_055762

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FoxJ3 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse FoxJ3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5786) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for FoxJ3 was detected at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxJ3

FoxJ3 (Forkhead box protein J3) is a 69 kDa (predicted) nuclear protein that is a Class 1 member of the Fox family of transcription factors. It is expressed in embryonic neuroectoderm, neural crest and somitic condensations, and is suggested to participate in terminal tissue differentiation. Human FoxJ3 is 622 amino acids (aa) in length. It contains one forkhead DNA binding region (aa 77‑168) plus a phosphorylation site at Ser223. There are two potential splice variants. One shows a deletion of aa 177‑210, while a second shows a 12 aa substitution for aa 177‑622. Over aa 375‑552, human FoxJ3 shares 93% aa identity with mouse FoxJ3.

Long Name

Forkhead Box J3

Entrez Gene IDs

22887 (Human); 230700 (Mouse); 313554 (Rat)

Alternate Names

forkhead box J3; forkhead box protein J3; FoxJ3; KIAA1041; MGC165036; MGC176686

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human/Mouse FoxJ3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1