Human/Mouse Frizzled-3 APC-conjugated Antibody

Name: Human/Mouse Frizzled-3 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB1001A
Price: 419 USD

Catalog #: FAB1001A Datasheet / COA / SDS

Discontinued Product

FAB1001A has been discontinued.

View all Frizzled-3 products.

Detection of Frizzled‑3 in HEK293 Human Cell Line by Flow Cytometry.

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Human/Mouse Frizzled-3 APC-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse Frizzled-3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Frizzled-1, -4, -7, or -8 is observed. By flow cytometry and immunohistochemistry, this antibody also detects mouse Frizzled-3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3. This antibody also detects Frizzled-3 on human HEK293 cells by immunocytochemistry and flow cytometry.

Source

Monoclonal Rat IgG_2A Clone # 169310

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-3
Met1-Val157
Accession # Q61086

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Frizzled‑3 in HEK293 Human Cell Line by Flow Cytometry. HEK293 human embryonic kidney cell line was stained with Rat Anti-Human/Mouse Frizzled-3 APC-conjugated Monoclonal Antibody (Catalog # FAB1001A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of Frizzled‑3 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Human/Mouse Frizzled-3 APC-conjugated Monoclonal Antibody (Catalog # FAB1001A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Frizzled-3

Frizzled-3 is an integral membrane protein that belongs to the Frizzled family of G-protein coupled receptors. Frizzleds function as receptors for Wnt proteins.

Entrez Gene IDs

7976 (Human); 14365 (Mouse)

Alternate Names

frizzled (Drosophila) homolog 3; frizzled homolog 3 (Drosophila); Frizzled3; Frizzled-3; Fz-3; FZD3; hFz3