Human/Mouse Frizzled-4 Biotinylated Antibody

Catalog #: BAM194
1 Citation Datasheet / COA / SDS

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BAM194

All Frizzled-4 Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Frizzled-4 Biotinylated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Frizzled-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse Frizzled-1, -2, -3, -6, -7, or -8 is observed. In flow cytometry and immunohistochemistry, this antibody also detected Frizzled-4 on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3, and also on human HEK293 cells.

Source

Monoclonal Rat IgG_2A Clone # 145901

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4
Met1-Glu180
Accession # Q61088

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Endotoxin Level

<0.15 EU per 1 μg of the antibody by the LAL method.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Frizzled-4 (Catalog # 5847-FZ)
Recombinant Mouse Frizzled-4 Fc Chimera (Catalog # 194-FZ)

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 human embryonic kidney cell line

Immunocytochemistry

8-25 µg/mL

Immersion fixed HEK293 human embryonic kidney cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-4

Frizzled-4 belongs to the Frizzled family of 7-pass transmembrane proteins and binds to Wnt-8. It is implicated in several developmental processes, notably in retinal angiogenesis.

Entrez Gene IDs

8322 (Human); 14366 (Mouse)

Alternate Names

CD344 antigen; CD344; EVR1; exudative vitreoretinopathy 1; FEVR; frizzled (Drosophila) homolog 4; frizzled homolog 4 (Drosophila); Frizzled4; Frizzled-4; Fz-4; FZD4; FZD4S; FzE4; GPCR; hFz4; MGC34390; WNT receptor frizzled-4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse Frizzled-4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Focal adhesion kinase splice variants maintain primitive acute myeloid leukemia cells through altered wnt signaling.
Authors: Despeaux M, Chicanne G, Rouer E, De Toni-Costes F, Bertrand J, Mansat-De Mas V, Vergnolle N, Eaves C, Payrastre B, Girault JA, Racaud-Sultan C
Stem Cells, 2012-08-01;30(8):1597-610.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry