Human/Mouse Ghrelin Antibody Summary
Gly24-Lys117
Accession # Q9EQX0
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Ghrelin in Mouse Stomach. Ghrelin was detected in immersion fixed frozen sections of mouse stomach using Rat Anti-Human/Mouse Ghrelin Monoclonal Antibody (Catalog # MAB8200) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining is shown at both 60x magnification (upper panel) and 20x magnification (lower panel). Staining was localized to cytoplasm and gastric endocrine cells, respectively. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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Ghrelin in Human Stomach. Ghrelin was detected in immersion fixed paraffin-embedded sections of human stomach using Rat Anti-Human/Mouse Ghrelin Monoclonal Antibody (Catalog # MAB8200) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ghrelin
GHRL (Ghrelin/Obestatin Prepropeptide) is a 117 aa precursor peptide that is processed into two chains, Ghrelin (aa24-51) and Obestatin (aa76-98). The 18 kDa prepropeptide is cleaved into proGhrelin, and then further processed into mature Ghrelin by prohormone convertases. Mature Ghrelin is modified with an N-octanoyl group on serine 3 which is required for receptor binding. Ghrelin and Obestatin are predominantly synthesized in the gastric mucosa. Ghrelin plays a role in growth factor release and appetite suppression as well as many other functions in a variety of organs. Obestatin is a putative hormone that has been suggested to have opposite effects on growth factor release and appetite as Ghrelin.
Product Datasheets
Citations for Human/Mouse Ghrelin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
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FOXO1 Is Present in Stomach Epithelium and Determines Gastric Cell Distribution
Authors: McKimpson WM, Kuo T, Kitamoto T et al.
Gastro Hep Advances
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Pancreatic Nonhormone Expressing Endocrine Cells in Children With Type 1 Diabetes
Authors: Md Moin AS, Cory M, Ong A et al.
J Endocr Soc
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Limited extent and consequences of pancreatic SARS-CoV-2 infection
Authors: Verena van der Heide, Sonia Jangra, Phillip Cohen, Raveen Rathnasinghe, Sadaf Aslam, Teresa Aydillo et al.
Cell Reports
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Expression of free fatty acid receptor 2 in normal and neoplastic tissues
Authors: Ruhnke, N;Beyer, AL;Kaemmerer, D;Sänger, J;Schulz, S;Lupp, A;
Experimental and molecular pathology
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
FOXO1 Is Present in Stomach Epithelium and Determines Gastric Cell Distribution
Authors: McKimpson WM, Kuo T, Kitamoto T et al.
Gastro Hep Advances
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Sequential progenitor states mark the generation of pancreatic endocrine lineages in mice and humans
Authors: XX Yu, WL Qiu, L Yang, YC Wang, MY He, D Wang, Y Zhang, LC Li, J Zhang, Y Wang, CR Xu
Cell Research, 2021-03-10;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Bariatric surgery induces a new gastric mucosa phenotype with increased functional glucagon-like peptide-1 expressing cells
Authors: L Ribeiro-Pa, AC Jarry, JB Cavin, A Willemetz, J Le Beyec, A Sannier, S Benadda, AL Pelletier, M Hourseau, T Léger, B Morlet, A Couvelard, Y Anini, S Msika, JP Marmuse, S Ledoux, M Le Gall, A Bado
Nature Communications, 2021-01-04;12(1):110.
Species: Rat
Sample Types: Whole Tissues
Applications: IHC -
Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals
Authors: T Yung, F Poon, M Liang, S Coquenlorg, EC McGaugh, CC Hui, MD Wilson, MC Nostro, TH Kim
Nat Commun, 2019-10-11;10(1):4647.
Species: Human, Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Neurogenin3 phosphorylation controls reprogramming efficiency of pancreatic ductal organoids into endocrine cells
Authors: R Azzarelli, S Rulands, S Nestorowa, J Davies, S Campinoti, S Gillotin, P Bonfanti, B Göttgens, M Huch, B Simons, A Philpott
Sci Rep, 2018-10-18;8(1):15374.
Species: Mouse
Sample Types: Organoids
Applications: IHC -
Neuropeptide Y expression marks partially differentiated ? cells in mice and humans
Authors: P Rodnoi, M Rajkumar, ASM Moin, SK Georgia, AE Butler, S Dhawan
JCI Insight, 2017-06-15;2(12):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Characterization of Non-hormone Expressing Endocrine Cells in Fetal and Infant Human Pancreas.
Authors: Moin ASM, Montemurro C, Zeng K et al.
Front Endocrinol (Lausanne)
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