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Human/Mouse GLI-1 Antibody

Catalog #: AF3455
18 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3455
AF3455-SP
Detection of Human GLI‑1 by Western Blot.
2 Images
Product Details
Citations (18)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse GLI-1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse GLI-1.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse GLI-1
Met1-Glu237
Accession # P47806
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human GLI-1 antibody by Western Blot. View Larger

Detection of Human GLI‑1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human GLI-1. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3455) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GLI-1 at approximately 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry GLI-1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

GLI‑1 in HeLa Human Cell Line. GLI-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Goat Anti-Human/Mouse GLI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3455) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLI-1

GLI-1 is a member of the Krupple family of zinc finger proteins. GLI-1 is activated by the sonic hedgehog pathway and influences transcription of a variety of target genes by binding to the consensus site 5’CGGGTGGTC3’. GLI-1 activation leads to cellular proliferation and anti-apoptotic activities based on target genes activated.

Long Name
Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]
Entrez Gene IDs
2735 (Human); 14632 (Mouse); 140589 (Rat)
Alternate Names
GLI family zinc finger 1; GLI1; GLI-1; glioma-associated oncogene family zinc finger 1; glioma-associated oncogene homolog 1 (zinc finger protein); Glioma-associated oncogene; GLIzinc finger protein GLI1; Oncogene GLI

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Citations for Human/Mouse GLI-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Actin polymerization controls cilia-mediated signaling
    Authors: Michael L. Drummond, Mischa Li, Eric Tarapore, Tuyen T.L. Nguyen, Baina J. Barouni, Shaun Cruz et al.
    Journal of Cell Biology
  2. HHIPL1, a Gene at the 14q32 Coronary Artery Disease Locus, Positively Regulates Hedgehog Signaling and Promotes Atherosclerosis
    Authors: Dimitra Aravani, Gavin E. Morris, Peter D. Jones, Helena K. Tattersall, Elisavet Karamanavi, Michael A. Kaiser et al.
    Circulation
  3. Timing of Smarcb1 and Nf2 inactivation determines schwannoma versus rhabdoid tumor development
    Authors: Vitte J, Gao F, Coppola G et al.
    Nat Commun
  4. Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair
    Authors: EC Jeffery, TLA Mann, JA Pool, Z Zhao, SJ Morrison
    Cell Stem Cell, 2022-10-21;29(11):1547-1561.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Vaccination-based immunotherapy to target profibrotic cells in liver and lung
    Authors: M Sobecki, J Chen, E Krzywinska, S Nagarajan, Z Fan, E Nelius, JM Monné Rodr, F Seehusen, A Hussein, G Moschini, EY Hajam, R Kiran, D Gotthardt, J Debbache, C Badoual, T Sato, T Isagawa, N Takeda, C Tanchot, E Tartour, A Weber, S Werner, J Loffing, L Sommer, V Sexl, C Münz, C Feghali-Bo, E Pachera, O Distler, J Snedeker, C Jamora, C Stockmann
    Cell Stem Cell, 2022-09-15;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Dhh signaling pathway regulates reconstruction of seminiferous tubule-like structure
    Authors: M Min, T Song, M Sun, T Wang, J Tan, J Zhang
    Reproductive biology, 2022-08-17;22(4):100684.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  7. Hedgehog-GLI signalling promotes chemoresistance through the regulation of ABC transporters in colorectal cancer cells
    Authors: A Po, A Citarella, G Catanzaro, ZM Besharat, S Trocchiane, F Gianno, C Sabato, M Moretti, E De Smaele, A Vacca, ME Fiori, E Ferretti
    Sci Rep, 2020-08-19;10(1):13988.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Identification of a novel tumor angiogenesis inhibitor targeting Shh/Gli1 signaling pathway in Non-small cell lung cancer
    Authors: X Lei, Y Zhong, L Huang, S Li, J Fu, L Zhang, Y Zhang, Q Deng, X Yu
    Cell Death Dis, 2020-04-14;11(4):232.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2017-11-13;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Mechanism of inhibition of the tumor suppressor Patched by Sonic Hedgehog
    Proc Natl Acad Sci USA, 2016-09-19;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. The hedgehog system machinery controls transforming growth factor-beta-dependent myofibroblastic differentiation in humans: involvement in idiopathic pulmonary fibrosis.
    Authors: Cigna N, Farrokhi Moshai E, Brayer S, Marchal-Somme J, Wemeau-Stervinou L, Fabre A, Mal H, Leseche G, Dehoux M, Soler P, Crestani B, Mailleux A
    Am J Pathol, 2012-09-29;181(6):2126-37.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. A novel synthetic smoothened antagonist transiently inhibits pancreatic adenocarcinoma xenografts in a mouse model.
    Authors: Strand MF, Wilson SR, Dembinski JL, Holsworth DD, Khvat A, Okun I, Petersen D, Krauss S
    PLoS ONE, 2011-06-15;6(6):e19904.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  13. Noncanonical hedgehog pathway activation through SRF-MKL1 promotes drug resistance in basal cell carcinomas
    Authors: RJ Whitson, A Lee, NM Urman, A Mirza, CY Yao, AS Brown, JR Li, G Shankar, MA Fry, SX Atwood, EY Lee, ST Hollmig, SZ Aasi, KY Sarin, MP Scott, EH Epstein, JY Tang, AE Oro
    Nat. Med., 2018-02-05;0(0):.
  14. Airway epithelial cell differentiation relies on deficient Hedgehog signalling in COPD
    Authors: R Belgacemi, E Luczka, J Ancel, Z Diabasana, JM Perotin, A Germain, N Lalun, P Birembaut, X Dubernard, JC Mérol, G Delepine, M Polette, G Deslée, V Dormoy
    EBioMedicine, 2019-12-23;51(0):102572.
  15. Altered bone growth dynamics prefigure craniosynostosis in a zebrafish model of Saethre-Chotzen syndrome
    Authors: Camilla S Teng, Man-chun Ting, D'Juan T Farmer, Mia Brockop, Robert E Maxson, J Gage Crump
    eLife
  16. Gli1 promotes epithelial–mesenchymal transition and metastasis of non-small cell lung carcinoma by regulating snail transcriptional activity and stability
    Authors: Xueping Lei, Zhan Li, Yihang Zhong, Songpei Li, Jiacong Chen, Yuanyu Ke et al.
    Acta Pharmaceutica Sinica B
  17. Sonic Hedgehog Is a Novel Tubule-Derived Growth Factor for Interstitial Fibroblasts after Kidney Injury
    Authors: Dong Zhou, Yingjian Li, Lili Zhou, Roderick J. Tan, Liangxiang Xiao, Min Liang et al.
    Journal of the American Society of Nephrology
  18. LAP2 Proteins Chaperone GLI1 Movement between the Lamina and Chromatin to Regulate Transcription
    Authors: Amar N. Mirza, Siegen A. McKellar, Nicole M. Urman, Alexander S. Brown, Tyler Hollmig, Sumaira Z. Aasi et al.
    Cell

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Reviews for Human/Mouse GLI-1 Antibody

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Human/Mouse GLI-1 Antibody
By Anonymous on 01/18/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Brain (cerebral cortex) Species: Mouse

Used antibody at 20ug/ml overnight at 4C in TBS+1% BSA. In image green=Gli1; blue=DAPI.

Immunocytochemistry/Immunofluorescence Human/Mouse GLI-1 Antibody AF3455