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Human/Mouse GLP/EHMT1 Antibody

Catalog #: PP-B0422-00
19 Citations 3 Reviews Datasheet / COA / SDS
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PP-B0422-00
Product Details
Citations (19)
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Reviews (3)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse GLP/EHMT1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
This antibody specifically recognizes mouse GLP and cross-reacts with human GLP. Not yet tested in other species.
Source
Monoclonal Mouse IgG2A Clone # B0422
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant mouse GLP/EHMT1
aa 134-234
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
This antibody can be used at 0.5 μg/mL with the appropriate secondary reagents to detect mouse GLP.
 
Immunohistochemistry
This antibody can be used at 5 μg/mL with the appropriate secondary reagents to detect mouse GLP.
 
Immunoprecipitation
Optimal dilutions should be determined by each laboratory.
 
Direct ELISA
This antibody can be used at 0.1 μg/mL with the appropriate secondary reagents to detect mouse GLP.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: GLP/EHMT1

Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic marker for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for murine embryogenesis. There is a single G9a-related methyltransferase in mammals, called GLP/Eu-HMTase1.

Long Name
G9a-like Protein 1/Euchromatic Histone-Lysine N-Methyltransferase 1
Entrez Gene IDs
79813 (Human); 77683 (Mouse)
Alternate Names
DEL9q34; EC 2.1.1.-; EC 2.1.1.43; EHMT1; euchromatic histone-lysine N-methyltransferase 1H3-K9-HMTase 5; Eu-HMTase1; Eu-HMTase1DEL9q34; EUHMTASE1FP13812; FLJ12879; G9a-like protein 1; GLP; GLP1; H3 lysine-9 specific 5; KIAA1876euchromatic histone methyltransferase 1; KMT1D; KMT1DDKFZp667M072; Lysine N-methyltransferase 1D

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Citations for Human/Mouse GLP/EHMT1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. TNF? Effects on Adipocytes Are Influenced by the Presence of Lysine Methyltransferases, G9a (EHMT2) and GLP (EHMT1)
    Authors: Able, AA;Richard, AJ;Stephens, JM;
    Biology
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Cross-Talk Between Histone Methyltransferases and Demethylases Regulate REST Transcription During Neurogenesis
    Authors: Jyothishmathi Swaminathan, Shinji Maegawa, Shavali Shaik, Ajay Sharma, Javiera Bravo-Alegria, Lei Guo et al.
    Frontiers in Oncology
  3. E2F6 initiates stable epigenetic silencing of germline genes during embryonic development
    Authors: T Dahlet, M Truss, U Frede, H Al Adhami, AF Bardet, M Dumas, J Vallet, J Chicher, P Hammann, S Kottnik, P Hansen, U Luz, G Alvarez, G Auclair, J Hecht, PN Robinson, C Hagemeier, M Weber
    Nature Communications, 2021-06-11;12(1):3582.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Two conserved epigenetic regulators prevent healthy ageing
    Authors: J Yuan, SY Chang, SG Yin, ZY Liu, X Cheng, XJ Liu, Q Jiang, G Gao, DY Lin, XL Kang, SW Ye, Z Chen, JA Yin, P Hao, L Jiang, SQ Cai
    Nature, 2020-02-26;0(0):.
    Species: Human
    Sample Types: Cells
    Applications: Immunoprecipitation
  5. G9a regulates temporal preimplantation developmental program and lineage segregation in blastocyst
    Authors: JJ Zylicz, M Borensztei, FC Wong, Y Huang, C Lee, S Dietmann, MA Surani
    Elife, 2018-05-10;7(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Epigenetic response to environmental stress: Assembly of BRG1–G9a/GLP–DNMT3 repressive chromatin complex on Myh6 promoter in pathologically stressed hearts☆,☆☆
    Authors: Pei Han, Wei Li, Jin Yang, Ching Shang, Chiou-Hong Lin, Wei Cheng et al.
    Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
  7. Co-repressor CBFA2T2 regulates pluripotency and germline development
    Authors: Shengjiang Tu
    Nature, 2016-06-08;534(7607):387-90.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development
    Authors: Jan J Zylicz, Sabine Dietmann, Ufuk Günesdogan, Jamie A Hackett, Delphine Cougot, Caroline Lee et al.
    eLife
  9. Chromatin landscape defined by repressive histone methylation during oligodendrocyte differentiation.
    Authors: Liu J, Magri L, Zhang F, Marsh N, Albrecht S, Huynh J, Kaur J, Kuhlmann T, Zhang W, Slesinger P, Casaccia P
    J Neurosci, 2015-01-07;35(1):352-65.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  10. G9a co-suppresses LINE1 elements in spermatogonia
    Authors: Monica Di Giacomo, Stefano Comazzetto, Srihari C Sampath, Srinath C Sampath, Dónal O’Carroll
    Epigenetics & Chromatin
  11. Inhibition of euchromatic histone methyltransferase 1 and 2 sensitizes chronic myeloid leukemia cells to interferon treatment.
    Authors: Loh S, Ng W, Yeo K, Lim Y, Ea C
    PLoS ONE, 2014-07-31;9(7):e103915.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. The methyltransferase G9a regulates HoxA9-dependent transcription in AML.
    Authors: Lehnertz B, Pabst C, Su L, Miller M, Liu F, Yi L, Zhang R, Krosl J, Yung E, Kirschner J, Rosten P, Underhill T, Jin J, Hebert J, Sauvageau G, Humphries R, Rossi F
    Genes Dev, 2014-02-15;28(4):317-27.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Distinct roles of KAP1, HP1 and G9a/GLP in silencing of the two-cell-specific retrotransposon MERVL in mouse ES cells
    Authors: Irina A Maksakova, Peter J Thompson, Preeti Goyal, Steven JM Jones, Prim B Singh, Mohammad M Karimi et al.
    Epigenetics & Chromatin
  14. EHMT1 protein binds to nuclear factor-kappaB p50 and represses gene expression.
    Authors: Ea C, Hao S, Yeo K, Baltimore D
    J Biol Chem, 2012-07-16;287(37):31207-17.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  15. The histone methyltransferase SETDB1 is recurrently amplified in melanoma and accelerates its onset.
    Authors: Ceol CJ, Houvras Y, Jane-Valbuena J, Bilodeau S, Orlando DA, Battisti V, Fritsch L, Lin WM, Hollmann TJ, Ferre F, Bourque C, Burke CJ, Turner L, Uong A, Johnson LA, Beroukhim R, Mermel CH, Loda M, Ait-Si-Ali S, Garraway LA, Young RA, Zon LI
    Nature, 2011-03-24;471(7339):513-7.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  16. Control of cognition and adaptive behavior by the GLP/G9a epigenetic suppressor complex.
    Authors: Schaefer A, Sampath SC, Intrator A
    Neuron, 2009-12-10;64(5):678-91.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. DNA methylation in ES cells requires the lysine methyltransferase G9a but not its catalytic activity.
    Authors: Dong K, Maksakova I, Mohn F, Leung D, Appanah R, Lee S, Yang H, Lam L, Mager D, Schubeler D, Tachibana M, Shinkai Y, Lorincz M
    EMBO J, 2008-09-25;27(20):2691-701.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  18. IFI16, a nuclear innate immune DNA sensor, mediates epigenetic silencing of herpesvirus genomes by its association with H3K9 methyltransferases SUV39H1 and GLP
    Authors: Roy A, Ghosh A, Kumar B, Chandran B
    Elife
  19. SPOC1 modulates DNA repair by regulating key determinants of chromatin compaction and DNA damage response
    Authors: Mund A, Schubert T, Staege H et al.
    Nucleic Acids Res

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Reviews for Human/Mouse GLP/EHMT1 Antibody

Average Rating: 4.3 (Based on 3 Reviews)

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Human/Mouse GLP/EHMT1 Antibody
By Anonymous on 09/07/2021
Application: IHC Sample Tested: Brain tissue Species: Human
Immunohistochemistry Human/Mouse GLP/EHMT1 Antibody PP-B0422-00
Human/Mouse GLP/EHMT1 Antibody
By Anonymous on 06/19/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: CCD-1070Sk human foreskin fibroblast cell line Species: Human
Immunocytochemistry/Immunofluorescence Human/Mouse GLP/EHMT1 Antibody PP-B0422-00
Human/Mouse GLP/EHMT1 Antibody
By Anonymous on 01/04/2018
Application: WB Sample Tested: RL human non-Hodgkin's lymphoma B cell line Species: Human
Western Blot Human/Mouse GLP/EHMT1 Antibody PP-B0422-00