Human/Mouse HAND2 Antibody Summary
Met1-Gln217
Accession # P61296
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of HAND2 in Mouse Embryo. HAND2 was detected in immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo using Goat Anti-Human/Mouse HAND2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3876) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei in developing tongue. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HAND2
HAND2 (also known as dHAND, Thing-2 and Hed) is a 24 kDa, class B member of the basic helix-loop-helix transcription factors superfamily. HAND2 is essential for cardiac morphogenesis and required for vascular development and regulation of angiogenesis, possibly through VEGF signaling. HAND2 is also implicated in limb development acting as an upstream regulator of sonic hedgehog. Alternative initiation and internal deletions result in at least three potential HAND2 isoforms. Human and mouse HAND2 proteins are 100% identical.
Product Datasheets
Citations for Human/Mouse HAND2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Integrated bioinformatics identifies the dysregulation induced by aberrant gene methylation in colorectal carcinoma
Authors: Ye Z, Li Y, Xie J et al.
Genes Dis
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Cardiac Reprogramming Factors Synergistically Activate Genome-wide Cardiogenic Stage-Specific Enhancers
Authors: H Hashimoto, Z Wang, GA Garry, VS Malladi, GA Botten, W Ye, H Zhou, M Osterwalde, DE Dickel, A Visel, N Liu, R Bassel-Dub, EN Olson
Cell Stem Cell, 2019-05-09;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Activin-like kinase 2 functions in peri-implantation uterine signaling in mice and humans.
Authors: Clementi C, Tripurani S, Large M, Edson M, Creighton C, Hawkins S, Kovanci E, Kaartinen V, Lydon J, Pangas S, DeMayo F, Matzuk M
PLoS Genet, 2013-11-14;9(11):e1003863.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
miR-1/133a Clusters Cooperatively Specify the Cardiomyogenic Lineage by Adjustment of Myocardin Levels during Embryonic Heart Development
Authors: Katharina Wystub, Johannes Besser, Angela Bachmann, Thomas Boettger, Thomas Braun
PLoS Genetics
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Distinct Roles of Hand2 in Initiating Polarity and Posterior Shh Expression during the Onset of Mouse Limb Bud Development
Authors: Antonella Galli, Dimitri Robay, Marco Osterwalder, Xiaozhong Bao, Jean-Denis Bénazet, Muhammad Tariq et al.
PLoS Genetics
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Antibody was used on E12.5 mouse sections (heart). Specific on Hand2+ Right Ventricle. Concentration used - 20ug/mL.
