Human/Mouse Hck Antibody

Catalog #: MAB3915
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3915
MAB3915-SP

Detection of Human/Mouse Hck by Western Blot.

1 Image

All Hck Antibodies

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Hck Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects endogenous human and mouse Hck at 59 kDa in Western blots.

Source

Monoclonal Rat IgG_2A Clone # 394903

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Hck
Met22-Asp80
Accession # P08631

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human/Mouse Hck by Western Blot. Western blot shows lysates of A20 mouse B cell lymphoma cell line, DA3 mouse myeloma cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Hck Monoclonal Antibody (Catalog # MAB3915) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Hck at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hck

Hemopoietic cell kinase (Hck) is a member of the Src family of tyrosine kinases. Hck expression is prominent in cells of myeloid lineage, particularly in granulocytes and monocytes, with protein levels increasing during terminal differentiation and macrophage activation. Hck contains a unique amino-terminal domain that is subject to lipid modifications, targeting this kinase to the plasma membrane.

Long Name

Hemopoietic Cell Kinase

Entrez Gene IDs

3055 (Human); 15162 (Mouse); 25734 (Rat)

Alternate Names

Bmk; EC 2.7.10; EC 2.7.10.2; Hck; Hctk; hemopoietic cell kinaseJTK9; JTK9; p59-HCK/p60-HCK; tyrosine-protein kinase HCK

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse Hck Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

A dominant function of LynB kinase in preventing autoimmunity
Authors: BF Brian, ML Sauer, JT Greene, SE Senevirath, AJ Lindstedt, OL Funk, BL Ruis, LA Ramirez, JL Auger, WL Swanson, MG Nunez, BS Moriarity, CA Lowell, BA Binstadt, TS Freedman
Science Advances, 2022-04-22;8(16):eabj5227.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot