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Human/Mouse HIF-1 alpha/HIF1A APC-conjugated Antibody

Catalog #: IC1935A
7 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC1935A
Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry.
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Citations (7)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse HIF-1 alpha/HIF1A APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Recognizes human and mouse HIF-1 alpha in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 241812
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human HIF-1 alpha
Arg575-Asn826
Accession # Q16665.1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of HIF-1a antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line treated with CoCl2was stained with Mouse Anti-Human/Mouse HIF-1a APC-conjugated Monoclonal Antibody (Catalog # IC1935A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: HIF-1 alpha/HIF1A

The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

Long Name
Hypoxia Inducible Factor 1 Subunit Alpha
Entrez Gene IDs
3091 (Human); 15251 (Mouse); 29560 (Rat)
Alternate Names
AINT; anti-HIF-1 alpha; anti-HIF1A; ARNT interacting protein; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF1 alpha; HIF-1 alpha; HIF1; HIF1A; HIF-1a; HIF-1alpha; HIF-1-alpha; HIF1-alpha; hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1-alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8

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Citations for Human/Mouse HIF-1 alpha/HIF1A APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Germinal center hypoxia in tumor-draining lymph nodes negatively regulates tumor-induced humoral immune responses in mouse models of breast cancer
    Authors: Firmino NS, Cederberg RA, Lee CM Et al.
    Oncoimmunology
  2. Itaconate ameliorates autoimmunity by modulating T cell imbalance via metabolic and epigenetic reprogramming
    Authors: K Aso, M Kono, M Kanda, Y Kudo, K Sakiyama, R Hisada, K Karino, Y Ueda, D Nakazawa, Y Fujieda, M Kato, O Amengual, T Atsumi
    Nature Communications, 2023-02-27;14(1):984.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity
    Authors: Agnieszka Chryplewicz, Julie Scotton, Mélanie Tichet, Anoek Zomer, Ksenya Shchors, Johanna A. Joyce et al.
    Cancer Cell
  4. Hypoxia induces HIF1alpha-dependent epigenetic vulnerability in triple negative breast cancer to confer immune effector dysfunction and resistance to anti-PD-1 immunotherapy
    Authors: S Ma, Y Zhao, WC Lee, LT Ong, PL Lee, Z Jiang, G Oguz, Z Niu, M Liu, JY Goh, W Wang, MA Bustos, S Ehmsen, A Ramasamy, DSB Hoon, HJ Ditzel, EY Tan, Q Chen, Q Yu
    Nature Communications, 2022-07-15;13(1):4118.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Uncoupling of macrophage inflammation from self-renewal modulates host recovery from respiratory viral infection
    Authors: Bibo Zhu, Yue Wu, Su Huang, Ruixuan Zhang, Young Min Son, Chaofan Li et al.
    Immunity
  6. Endogenous oxidized phospholipids reprogram cellular metabolism and boost hyperinflammation
    Authors: M Di Gioia, R Spreafico, JR Springstea, MM Mendelson, R Joehanes, D Levy, I Zanoni
    Nat. Immunol., 2019-11-25;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor
    Authors: Q Li, D Li, X Zhang, Q Wan, W Zhang, M Zheng, L Zou, C Elly, JH Lee, YC Liu
    Immunity, 2018-02-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human/Mouse HIF-1 alpha/HIF1A APC-conjugated Antibody

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Human/Mouse HIF-1 alpha/HIF1A APC-conjugated Antibody
By Anonymous on 04/03/2021
Application: Flow Sample Tested: IC-21 mouse macrophage cell line,macrophage Species: Human
Flow Cytometry Human/Mouse HIF-1 alpha/HIF1A APC-conjugated Antibody IC1935A
Human/Mouse HIF-1 alpha APC-conjugated Antibody
By Anonymous on 08/03/2016
Application: Flow Sample Tested: T regulatory cells Species: Mouse
Flow Cytometry Human/Mouse HIF-1 alpha APC-conjugated Antibody IC1935A