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Human/Mouse IRF9 Antibody

Catalog #: AF5629
2 Citations Datasheet / COA / SDS

Discontinued Product

AF5629 has been discontinued.
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Detection of Human IRF9 by Western Blot.
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Citations (2)
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Human/Mouse IRF9 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects endogenous human and mouse IRF9 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IRF9
Ser238-Val393
Accession # Q00978
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IRF9 antibody by Western Blot. View Larger

Detection of Human IRF9 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and 3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse IRF9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5629) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IRF9 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRF9

IRF9 (interferon regulatory factor 9; also ISGF3-gamma ) is a 48 kDa member of the IRF family of proteins. It is widely expressed, and serves as a component of the ISGF3 complex. Following activation of IFNAR by IFN-alpha / beta, IRF9 is acetylated by CBP on Lys81. IRF9 then associates with activated STAT1 and STAT2 to form an ISGF3 complex that is translocated into the nucleus. Human IRF9 is 393 amino acids (aa) in length. It contains an N-terminal DNA-binding region (aa 11‑112) that contains an NLS (aa 66‑85), plus a STAT-binding domain (aa 200‑393). There are two potential isoform variants. One shows a deletion of aa 218‑331 and 340‑393, while another shows a five aa substitution for aa 217‑393. Over aa 238‑393, human IRF9 shares 79% aa identity with mouse IRF9.

Long Name
Interferon Regulatory Factor 9
Entrez Gene IDs
10379 (Human); 16391 (Mouse); 305896 (Rat)
Alternate Names
IFN-alpha-responsive transcription factor subunit; interferon regulatory factor 9; Interferon-stimulated gene factor 3 gamma; interferon-stimulated transcription factor 3, gamma (48kD); interferon-stimulated transcription factor 3, gamma 48kDa; IRF9; IRF-9; ISGF3 p48 subunit; ISGF3; ISGF3G; ISGF3GISGF-3 gamma; p48; Transcriptional regulator ISGF3 subunit gamma

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Citations for Human/Mouse IRF9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Specific enhancer selection by IRF3, IRF5�and IRF9 is determined by ISRE half-sites, 5' and 3' flanking bases, collaborating transcription factors and the chromatin environment in a combinatorial fashion
    Authors: M Csumita, A Csermely, A Horvath, G Nagy, F Monori, L Göczi, HA Orbea, W Reith, L Széles
    Nucleic Acids Res., 2020-01-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ChIP
  2. Critical role of the IFN-stimulated gene factor 3 complex in TLR-mediated IL-27p28 gene expression revealing a two-step activation process.
    Authors: Molle C, Goldman M, Goriely S
    J. Immunol., 2010-01-18;184(4):1784-92.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot

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