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Human/Mouse MSK2 Antibody

Catalog #: MAB2310
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2310
MAB2310-SP
Detection of Human/Mouse MSK2 by Western Blot.
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Product Details
Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse MSK2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse MSK2 in Western blots. Does not detect recombinant human MSK1.
Source
Monoclonal Rat IgG2A Clone # 261034
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MSK2
Met1-Ser772
Accession # O75676
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse MSK2 antibody by Western Blot. View Larger

Detection of Human/Mouse MSK2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, DU145 human prostate carcinoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse MSK2 Monoclonal Antibody (Catalog # MAB2310) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human MSK1 and MSK2 (2 ng/lane) were included. A specific band for MSK2 was detected at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSK2

Mitogen- and stress-activated protein kinase 2 (MSK2), also known as ribosomal protein S6 kinase B (RSKB) and RPS6KA4, belongs to the family of AGC kinases. Both MSK2 and the related MSK1 have two kinase domains connected by a regulatory linker region and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB.

Long Name
Mitogen- and Stress-activated Protein Kinase 2
Entrez Gene IDs
8986 (Human); 56613 (Mouse)
Alternate Names
MSK2; ribosomal protein S6 kinase, 90kDa, polypeptide 4; RPS6KA4; RSKB

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Citations for Human/Mouse MSK2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. miR-93 regulates Msk2-mediated chromatin remodelling in diabetic nephropathy
    Authors: Shawn S. Badal, Yin Wang, Jianyin Long, David L. Corcoran, Benny H. Chang, Luan D. Truong et al.
    Nature Communications
  2. A Signaling Crosstalk Links SNAIL to the 37/67 kDa Laminin-1 Receptor Ribosomal Protein SA and Regulates the Acquisition of a Cancer Stem Cell Molecular Signature in U87 Glioblastoma Neurospheres
    Authors: L Gresseau, ME Roy, S Duhamel, B Annabi
    Cancers, 2022-11-30;14(23):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Mitogen- and stress-activated protein kinase 2 and cyclic AMP response element binding protein are activated in lesional psoriatic epidermis.
    Authors: Funding AT, Johansen C, Kragballe K, Iversen L
    J. Invest. Dermatol., 2007-04-12;127(8):2012-9.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  4. Protein kinase Msk1 physically and functionally interacts with the KMT2A/MLL1 methyltransferase complex and contributes to the regulation of multiple target genes
    Authors: Maaike Wiersma, Marianne Bussiere, John A. Halsall, Nil Turan, Robert Slany, Bryan M. Turner et al.
    Epigenetics & Chromatin

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