Human/Mouse MYF-5 Antibody

Catalog #: AF4027
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4027
AF4027-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse MYF-5 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects recombinant human MYF-5 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human MYF-5
Met1-Leu255
Accession # P13349

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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MYF‑5 in C2C12 Mouse Cell Line. MYF-5 was detected in immersion fixed C2C12 mouse myoblast cell line undifferentiated (upper panel) or differentiated with horse serum for 7 days (lower panel) using Goat Anti-Human/Mouse MYF-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4027) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MYF-5

MYF-5 (Myogenic factor 5; also bHLHc2) is a 37-39 kDa transcriptional activator that belongs to the bHLH superfamily of myogenic regulatory factors. It is found in embryonic skeletal muscle progenitors and neurons, and is induced by SHH and Wnt1. Expression of MYF-5 protein prompts nonmuscle cells to adopt a muscle precursor phenotype. Notably, neurons appear to block MYF-5 accumulation, thus preserving their unique phenotype. MYF-5 forms heterodimers with multiple molecules. When complexed to I-mfa, MYF-5 is retained in the cytoplasm and is inactive; when complexed to E12, MYF-5 is transcriptionally active. Phosphorylation regulates MYF-5 stability. Human MYF-5 is 255 amino acids (aa) in length. It contains one myogenic basic region (aa 1-83) followed by an HLH motif (aa 96-135). Full-length human MYF-5 (aa 1-255) shares 89% aa sequence identity with mouse MYF-5.

Long Name

Myogenic Regulatory Factor 5

Entrez Gene IDs

4617 (Human); 17877 (Mouse); 299766 (Rat)

Alternate Names

bHLHc2; bHLHc2Class C basic helix-loop-helix protein 2; MYF5; MYF-5; myogenic factor 5

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse MYF-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State
Authors: L Zhang, J Avery, A Yin, AM Singh, TS Cliff, H Yin, S Dalton
Cell Stem Cell, 2020-08-11;0(0):.
Species: Human
Sample Types: Cell Lysates, Organoid, Whole Cells
Applications: ICC, Flow Cytometry, IHC, Western Blot