Human/Mouse Netrin-4 Antibody

Catalog #: MAB1132 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1132
MAB1132-SP

All Netrin-4 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Netrin-4 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Netrin‑4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) Netrin-1, rmNetrin-G1a, rmNetrin-G2a, recombinant human Netrin-4, recombinant chicken (rch) Netrin-1, or rchNetrin‑2 is observed.

Source

Monoclonal Rat IgG_2A Clone # 299612

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Netrin-4
Gly19-Val628
Accession # Q9JI33

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Netrin-4 (Catalog # 1254-N4)
Recombinant Mouse Netrin-4 (Catalog # 1132-N4)
under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Netrin-4

Netrins are a family of laminin-related small proteins that are involved in neurite outgrowth and axon guidance. Netrins bind to the DCC and UNC5 family of receptors to attract or repel axons.

Entrez Gene IDs

59277 (Human); 57764 (Mouse)

Alternate Names

beta-Netrin; hepar-derived netrin-like protein; netrin 4; Netrin4; Netrin-4; NTN4; PRO3091