Human/Mouse Nodal Antibody Summary
His245-Leu354
Accession # P43021
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nodal
Nodal is a secreted protein that is a member of the Transforming Growth Factor-beta (TGF-beta ) superfamily. Nodal was named for its localized expression in the mouse node during gastrulation, and is first detected in the early primitive streak during mesoderm formation. Expression of the Nodal gene occurs asymmetrically in the left, but not right, lateral plate during somitogenesis. Nodal proteins play crucial roles in mesoderm formation and both anterior-posterior and left-right axis formation during vertebrate development. Members of the Nodal gene family include mouse Nodal, chick cNR-1, frog Xnr1-4, and zebrafish cyclops. Biologically active Nodal is a disulfide-linked homodimer and contains all seven of the cysteine residues necessary for formation of the “cysteine knot” characteristic of TGF-beta -related molecules. Mouse Nodal is 34 - 39% homologous in the conserved region to other TGF-beta superfamily members. Nodal has been shown to signal through a mechanism related to the Activin pathway, and signaling is mediated through both Smad2 and 3. Nodal signaling utilizes type II Activin receptors, together with ALK4/ActRIB, or the orphan type I receptor ALK7. Nodal interacts extracellularly with members of other protein families, including Cerberus, Lefty, and EGF-CFC ligands, such as Cripto. While the Cerberus and Lefty families act as Nodal antagonists, the EGF-CFC molecules act as co-receptors to facilitate Nodal signaling. The resulting concert of regulated Nodal activity allows for the precise control of mesoderm formation, neural patterning, and axis positioning and patterning during early vertebrate development.
- Kumar, A. et al. (2001) J. Biol. Chem. 276:656.
- Reissmann, E. et al. (2001) Genes & Dev. 15: 2010.
- Schier, A. and M. Shen (1999) Nature 403:385.
- Zhou, X. et al. (1993) Nature 361:543.
Product Datasheets
Citation for Human/Mouse Nodal Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Expression of nodal signalling components in cycling human endometrium and in endometrial cancer.
Authors: Papageorgiou I, Nicholls PK, Wang F
Reprod. Biol. Endocrinol., 2009-10-29;7(0):122.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
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This Nodal ab is much better than the other Nodal antibodies we have used. To see the quality of it, we run a western with R&D Systems purified Nodal protein (made in E.Coli). It is definitely not the best antibody, but not the worst either...
