Human/Mouse Orexin R2/HCRTR2 APC-conjugated Antibody

Name: Human/Mouse Orexin R2/HCRTR2 APC-conjugated Antibody
Brand: R&D Systems
SKU: IC5246A

Catalog #: IC5246A Datasheet / COA / SDS

Newer Version Available: IC5246R

NEW

Discontinued Product

IC5246A has been discontinued and is replaced by IC5246R.

Detection of Orexin R2/HCRTR2 in A172 Human Cell Line by Flow Cytometry.

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All Orexin R2/HCRTR2 Antibodies

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Human/Mouse Orexin R2/HCRTR2 APC-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Orexin R2/HCRTR2. Stains human and mouse Orexin R2/HCRTR2 transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2A Clone # 456738

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Orexin R2/HCRTR2
Met1-Trp444
Accession # AAC39602

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Orexin R2/HCRTR2 antibody in A172 Human Cell Line antibody by Flow Cytometry.

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Detection of Orexin R2/HCRTR2 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human/Mouse Orexin R2/HCRTR2 APC-conjugated Monoclonal Antibody (Catalog # IC5246A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry

Detection of Orexin R2/HCRTR2 antibody in Neuro-2A Mouse Cell Line antibody by Flow Cytometry.

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Detection of Orexin R2/HCRTR2 in Neuro‑2A Mouse Cell Line by Flow Cytometry. Neuro-2A mouse neuroblastoma cell line was stained with Mouse Anti-Human/Mouse Orexin R2/HCRTR2 APC-conjugated Monoclonal Antibody (Catalog # IC5246A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Orexin R2/HCRTR2

Hypocretin Receptor 2 (HCRTR2), also known as Orexin Receptor 2 or OX2R, is a 40 kDa 7-transmembrane G-protein-coupled glycoprotein that is a high affinity receptor for Orexins A and B (Hypocretins 1 and 2). In mouse brain, engagement of HCRTRs promotes wakefulness, such that absence of either orexins or their receptors creates a narcolepsy-like state. It also influences reward circuits involving food or addictive drugs. The extracellular portions of human HCRTR2 share 92% and 93% aa identity with corresponding portions of mouse and rat HCRTR2, respectively.

Long Name

Hypocretin Receptor Type 2

Entrez Gene IDs

3062 (Human); 387285 (Mouse); 25605 (Rat)

Alternate Names

HCRTR2; hypocretin (orexin) receptor 2; Hypocretin receptor type 2; Orexin R2; Orexin Receptor 2; orexin receptor type 2; OrexinR2; OX2R; Ox-2-R; ox2-R