Human/Mouse Patched 1/PTCH
(First Extracellular Loop) Antibody

Catalog #: MAB41051
10 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB41051
MAB41051-SP

Detection of Mouse Patched 1/PTCH by Western Blot.

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Product Details

Citations (10)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Patched 1/PTCH
(First Extracellular Loop) Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse Patched 1/PTCH (First Extracellular Loop) in direct ELISAs and Western blots. In direct ELISAs and Western blots, 15% cross‑reactivity with recombinant human Patched 2 is observed.

Source

Monoclonal Rat IgG_2A Clone # 413220

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Patched 1/PTCH
Glu108-Asp422
Accession # Q61115

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Patched 1/PTCH by Western Blot. Western blot shows lysates of MEF mouse embryonic feeder cells, Neuro-2A mouse neuroblastoma cell line, and mouse brain tissue. PVDF Membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Patched 1/PTCH (First Extracellular Loop) Monoclonal Antibody (Catalog # MAB41051) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Patched 1/PTCH at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Patched in Mouse Spinal Cord. Patched was detected in immersion fixed frozen sections of mouse spinal cord using 10 µg/mL Rat Anti-Human/Mouse Patched 1/PTCH (First Extracellular Loop) Monoclonal Antibody (Catalog # MAB41051) overnight at 4 °C. Tissue was stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry

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Patched 1/PTCH in Human Esophageal Squamous Cell Carcinoma. Patched 1/PTCH was detected in immersion fixed paraffin-embedded sections of human esophogeal squamous cell carcinoma tissue using Rat Anti-Human/Mouse Patched 1/PTCH (First Extracellular Loop) Monoclonal Antibody (Catalog # MAB41051) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Patched 1/PTCH

Patched homolog 1 (PTCH, also called PTC, PTC1 or PTCH1) is a 170‑175 kDa member of the Patched family of proteins. It is found on multiple cells types and forms a constitutive, inactivating complex with Smoothened. Upon SHH binding to PTC1, Smoothened is released and initiates downstream signaling. Mouse PTCH is a 12 transmembrane, 1434 aa glycoprotein that contains two terminal cytoplasmic domains. Only one mRNA species for PTCH has been reported in mice. However, it would appear likely that two or more splice forms exist, with variation occurring over the first 150 aa of the N-terminus. Over aa 108‑422, mouse PTCH shares 97% and 99% aa sequence identity with human and rat PTCH, respectively.

Entrez Gene IDs

5727 (Human); 19206 (Mouse); 89830 (Rat)

Alternate Names

BCNS; BCNSFLJ42602; FLJ26746; HPE7; mes; NBCCS; patched (Drosophila) homolog; Patched 1; patched homolog (Drosophila); patched homolog 1 (Drosophila); patched homolog 1; protein patched homolog 1; PTC; ptc1; PTCH protein +12b; PTCH protein +4'; PTCH protein -10; PTCH; PTCH1; PTCH11

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Hedgehog Family

Citations for Human/Mouse Patched 1/PTCH
(First Extracellular Loop) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Timing of Smarcb1 and Nf2 inactivation determines schwannoma versus rhabdoid tumor development
Authors: Vitte J, Gao F, Coppola G et al.
Nat Commun
NFAT5/TonEBP controls early acquisition of notochord phenotypic markers, collagen composition, and sonic hedgehog signaling during mouse intervertebral disc embryogenesis
Authors: Tessier S, Madhu V, Johnson ZI et al.
Dev. Biol.
Multiplatform molecular profiling uncovers two subgroups of malignant peripheral nerve sheath tumors with distinct therapeutic vulnerabilities
Authors: Suppiah, S;Mansouri, S;Mamatjan, Y;Liu, JC;Bhunia, MM;Patil, V;Rath, P;Mehani, B;Heir, P;Bunda, S;Velez-Reyes, GL;Singh, O;Ijad, N;Pirouzmand, N;Dalcourt, T;Meng, Y;Karimi, S;Wei, Q;Nassiri, F;Pugh, TJ;Bader, GD;Aldape, KD;Largaespada, DA;Zadeh, G;
Nature communications
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot
Human theca arises from ovarian stroma and is comprised of three discrete subtypes
Authors: Nicole Lustgarten Guahmich, Limor Man, Jerry Wang, Laury Arazi, Eleni Kallinos, Ariana Topper-Kroog et al.
Communications Biology
Persistent Properties of a Subpopulation of Cancer Cells Overexpressing the Hedgehog Receptor Patched
Authors: ÁJ Feliz More, A Hasanovic, A Morin, C Prunier, V Magnone, K Lebrigand, A Aouad, S Cogoluegne, J Favier, C Pasquier, I Mus-Veteau
Pharmaceutics, 2022-05-05;14(5):.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, ICC, Western Blot
Inhibition of Gli2 suppresses tumorigenicity in glioblastoma stem cells derived from a de novo murine brain cancer model
Authors: S Tanigawa, M Fujita, C Moyama, S Ando, H Ii, Y Kojima, T Fujishita, M Aoki, H Takeuchi, T Yamanaka, Y Takahashi, N Hashimoto, S Nakata
Cancer Gene Therapy, 2021-01-07;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Wnt Signaling Separates the Progenitor and Endocrine Compartments during Pancreas Development
Authors: N Sharon, J Vanderhoof, J Straubhaar, J Mueller, R Chawla, Q Zhou, EN Engquist, C Trapnell, DK Gifford, DA Melton
Cell Rep, 2019-05-21;27(8):2281-2291.e5.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Formation of the sacrum requires down-regulation of sonic hedgehog signaling in the sacral intervertebral discs
Authors: Raffaella Bonavita, Kathleen Vincent, Robert Pinelli, Chitra Lekha Dahia
Biology Open
Statins Synergize with Hedgehog Pathway Inhibitors for Treatment of Medulloblastoma
Authors: Renata E. Gordon, Li Zhang, Suraj Peri, Yin-Ming Kuo, Fang Du, Brian L. Egleston et al.
Clinical Cancer Research
Defective Wnt-dependent cerebellar midline fusion in a mouse model of Joubert syndrome.
Authors: Lancaster MA, Gopal DJ, Kim J
Nat. Med., 2011-05-29;17(6):726-31.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr