Human/Mouse Peroxiredoxin 3 Antibody

Detection of Human and Mouse Peroxiredoxin 3 by Western Blot. Western blot shows lysates of TF-1 human erythroleukemic cell line and mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Human/Mouse Peroxiredoxin 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6610) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Peroxiredoxin 3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Mouse Peroxiredoxin 3 by Western Blot Pharmacologic inhibition or genetic ablation of NOX reduces mitochondrial superoxide production in isolated CMs cultured in the presence of EtOH and ACA.(A) Scheme depicting incubation and imaging of isolated adult CMs from B6, ALDH2−/− or ALDH2−/−/gp91phox−/− mice with apocynin (300 μM), EtOH (100 μM) or ACA (100 μM). (B) MitoSOX-enhanced fluorescence per CMs. Quantification of 205–931 CMs on 6–12 independent low power fields per group. (C) Representative fluorescence images of CMs loaded with MitoSOX after culture for 18h in control media (leftmost column) in the presence of ACA 100 μM (middle column) and in the presence of ACA (100 μM) together with the NOX inhibitor apocynin (300 μM) rightmost column. (D) Peroxiredoxin 3 ratio monomer/dimer determined by Western blot under non-reducing, denaturing conditions in n = 4–7 independent samples per group. (E) Representative original Western blots. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep32554), licensed under a CC-BY license. Not internally tested by R&D Systems.