Home / STAT3 / Human/Mouse Phospho-STAT3 (Y705) DuoSet IC ELISA

Human/Mouse Phospho-STAT3 (Y705) DuoSet IC ELISA

Catalog #: DYC4607B-2
9 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DYC4607B-5
DYC4607B-2
Ancillary Products Available
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
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Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (1)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human/Mouse Phospho-STAT3 (Y705) DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4 hours 40 minutes (after plate preparation)
Sample Volume Required
Cell lysates (100 µL)
Assay Range
2344.0 - 150,000 pg/mL
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure phosphorylated human and mouse STAT3 (Y705) in cell lysates. An immobilized capture antibody specific for STAT3 binds both phosphorylated and unphosphorylated STAT3. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: STAT3

The STAT family of transcription factors, first described as key mediators of interferon signal transduction, are now recognized as signaling factors downstream of many cytokines, growth factors, and hormones. At least seven STATs exist: STAT1, 2, 3, 4, 5a, 5b, and 6. They are responsible for an array of cellular activities including regulating growth, survival, differentiation, motility, and the immune response. They are characterized structurally by the presence of the Src homology 2 (SH2) domain, and a C-terminal tyrosine phosphorylation site. Upon ligand binding, they are recruited to the activated receptor, via their SH2 domains, to receptor-associated tyrosine kinases of the Janus kinase family (JAK). Once phosphorylated by JAK, STATS are free to hetero- or homo-dimerize, and translocate to the nucleus where they bind DNA to regulate gene transcription.

Long Name:
Signal Transducer and Activator of Transcription 3
Entrez Gene IDs:
6774 (Human); 20848 (Mouse); 25125 (Rat)
Alternate Names:
Acute-phase response factor; APRFMGC16063; DNA-binding protein APRF; FLJ20882; HIES; signal transducer and activator of transcription 3 (acute-phase responsefactor); signal transducer and activator of transcription 3; STAT3

Citations for Human/Mouse Phospho-STAT3 (Y705) DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. ATP6V0D1 promotes alkaliptosis by blocking STAT3-mediated lysosomal pH homeostasis
    Authors: F Chen, S Zhu, R Kang, D Tang, J Liu
    Cell Reports, 2022-12-29;42(1):111911.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Single-cell-level protein analysis revealing the roles of autoantigen-reactive B lymphocytes in autoimmune disease and the murine model
    Authors: T Fukasawa, A Yoshizaki, S Ebata, A Yoshizaki-, Y Asano, A Enomoto, K Miyagawa, Y Kazoe, K Mawatari, T Kitamori, S Sato
    Elife, 2021-12-02;10(0):.
    Species: Human
    Sample Types: Cell Lysates
  3. miR-21-3p/IL-22 Axes Are Major Drivers of Psoriasis Pathogenesis by Modulating Keratinocytes Proliferation-Survival Balance and Inflammatory Response
    Authors: F Abdallah, E Henriet, A Suet, A Arar, R Clemençon, JM Malinge, G Lecellier, P Baril, C Pichon
    Cells, 2021-09-26;10(10):.
    Species: Human
    Sample Types: Cell Lysates
  4. Microfluidic Single-Cell Proteomics Assay Chip: Lung Cancer Cell Line Case Study
    Authors: Y Jung, M Son, YR Nam, J Choi, JR Heath, S Yang
    Micromachines, 2021-09-23;12(10):.
    Species: Human
    Sample Types: Cell Lysates
  5. Identification of a Novel Bone Marrow Cell-Derived Accelerator of Fibrotic Liver Regeneration Through Mobilization of Hepatic Progenitor Cells in Mice
    Authors: T Yanagawa, H Sumiyoshi, K Higashi, S Nakao, R Higashiyam, H Fukumitsu, K Minakawa, Y Chiba, Y Suzuki, K Sumida, K Saito, A Kamiya, Y Inagaki
    Stem Cells, 2018-10-31;37(1):89-101.
    Species: Mouse
    Sample Types: Cell Lysates
  6. Upregulation of SOCS3 in lung CD4+ T cells in a mouse model of chronic PA lung infection and suppression of Th17?mediated neutrophil recruitment in exogenous SOCS3 transfer in vitro
    Authors: FM Ding, RM Liao, YQ Chen, GG Xie, PY Zhang, P Shao, M Zhang
    Mol Med Rep, 2017-05-25;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Metformin inhibits the inflammatory response associated with cellular transformation and cancer stem cell growth.
    Proc Natl Acad Sci U S A, 2012-12-31;110(3):972-7.
    Species: Human
    Sample Types: Cell Lysates
  8. Microarray-based kinetic colorimetric detection for quantitative multiplex protein phosphorylation analysis.
    Authors: Holenya P, Kitanovic I, Heigwer F, Wolfl S
    Proteomics, 2011-04-18;11(10):2129-33.
    Species: Human
    Sample Types: Recombinant Protein
  9. IFN-alpha blocks IL-17 production by peripheral blood mononuclear cells in Behcet's disease.
    Authors: Liu X, Yang P, Wang C, Li F, Kijlstra A
    Rheumatology (Oxford), 2010-11-08;50(2):293-8.
    Species: Human
    Sample Types: Cell Lysates

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Human/Mouse Phospho-STAT3 (Y705) DuoSet IC ELISA
By Anonymous on 10/26/2018
Sample Tested: Stimulated Human Bronchial Epithelial Cell Line (BEAS-2B)
Human/Mouse Phospho-STAT3 (Y705) DuoSet IC ELISA DYC4607B-2