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Human/Mouse PIWIL1/HIWI Antibody

Catalog #: AF6548
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6548
AF6548-SP
PIWIL1/HIWI in Mouse Testes.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse PIWIL1/HIWI Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human PIWIL1/HIWI in direct ELISAs.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PIWIL1/HIWI
Arg82-Thr290
Accession # Q96J94
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry PIWIL1/HIWI antibody in Mouse Testes by Immunohistochemistry (IHC-Fr). View Larger

PIWIL1/HIWI in Mouse Testes. PIWIL1/HIWI was detected in perfusion fixed frozen sections of mouse testes using Goat Anti-Human PIWIL1/HIWI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6548) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of spermatocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PIWIL1/HIWI

PIWI (P-element-induced wimpy testis; also HIWI and PIWIL1) is a 92-98 kDa member of the Piwi subfamily, Argonaute family of proteins. It is expressed in germline cells, particularly spermatocytes and spermatids, and also appears in CD34+ hematopoietic stem cells plus select tumor types. PIWI binds to a series of 28‑34 nucleic acid long noncoding RNAs that participate in gametogenesis. It does so under the influence of Arg methyltransferases, and in conjunction with Tudor‑containing proteins. Human PIWI/PIWIL1 is 861 amino acids (aa) in length. It contains a methylation target region in the N-terminus, followed by an RNA-binding PAZ domain (aa 277-391) and an RNA-cleaving PIWI domain (aa 555-847). There are two isoform variants. One is widely expressed and shows a three aa substitution for aa 1‑89. The second contains a six aa substitution for aa 824-861. Over aa 82-290, human PIWI (HIWI) shares 95% aa sequence identity with mouse PIWI (MIWI).

Long Name
Piwi-like Protein 1
Entrez Gene IDs
9271 (Human)
Alternate Names
HIWI; HIWIpiwi homolog; MIWI; piwi (Drosophila)-like 1; PIWI; PIWIL1; piwi-like 1 (Drosophila); piwi-like protein 1

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Citations for Human/Mouse PIWIL1/HIWI Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Biopathological Significance of PIWI-piRNA Pathway Deregulation in Invasive Breast Carcinomas
    Authors: D Meseure, S Vacher, S Boudjemaa, M Laé, A Nicolas, R Leclere, W Chemlali, G Champenois, A Schnitzler, L Lesage, T Dubois, I Bieche
    Cancers (Basel), 2020-09-30;12(10):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Donor spermatogenesis in de novo formed seminiferous tubules from transplanted testicular cells in rhesus monkey testis.
    Authors: Shetty G, Mitchell JM, Lam TNA et al.
    Hum. Reprod.
  3. YTHDC2 serves a distinct late role in spermatocytes during germ cell differentiation
    Authors: AS Bailey, MT Fuller
    bioRxiv : the preprint server for biology, 2023-01-23;0(0):.

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