Human/Mouse Plexin A1 Antibody

Detection of Mouse Plexin A1 by Western Blot. Western blot shows lysates of mouse embryonic heart tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Plexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4309) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Plexin A1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Plexin A1 in Human Dendritic Cells. Plexin A1 was detected in immersion fixed immature human dendritic cells using Goat Anti-Mouse Plexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4309) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Plexin A1 in Mouse Spinal Cord. Plexin A1 was detected in immersion fixed frozen sections of embryonic mouse spinal cord using Mouse Plexin A1 Affinity-purified Polyclonal Antibody (Catalog # AF4309) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse Plexin A1 by Western Blot Localization of PlexinA1 and Npn1 in the coronal sections of WT and PlexinA1 KO brains at E16.5 and E17.5.(A) Immunohistochemistry with anti-PlexinA1 antibody revealed the localization of PlexinA1 only in WT brains at E16.5 and E17.5 (a, b) as well as the lack of PlexinA1 in PlexinA1 KO brains (c, d). Scale bars: 200 μm. (B, C) Western blot analysis using anti-PlexinA1 antibodies was performed with tissue lysates from the medial regions covering the cingulate cortex, axons from the cingulate and neocortex, and the CC of E16.5 and E17.5 brains. PlexinA1 protein was detected in WT brains (B; E16.5, C; E17.5) but not in PlexinA1 KO brains (B; E16.5, C; E17.5). (D) Immunohistochemistry with anti-Npn1 antibodies revealed localization of Npn1 in both WT (a; E16.5, b; E17.5) and PlexinA1 KO (c; E16.5, d; E17.5) brains at E16.5 and E17.5. Scale bars: 200 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31430342), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Plexin A1 by Western Blot Localization of PlexinA1 and Npn1 in the coronal sections of WT and PlexinA1 KO brains at E16.5 and E17.5.(A) Immunohistochemistry with anti-PlexinA1 antibody revealed the localization of PlexinA1 only in WT brains at E16.5 and E17.5 (a, b) as well as the lack of PlexinA1 in PlexinA1 KO brains (c, d). Scale bars: 200 μm. (B, C) Western blot analysis using anti-PlexinA1 antibodies was performed with tissue lysates from the medial regions covering the cingulate cortex, axons from the cingulate and neocortex, and the CC of E16.5 and E17.5 brains. PlexinA1 protein was detected in WT brains (B; E16.5, C; E17.5) but not in PlexinA1 KO brains (B; E16.5, C; E17.5). (D) Immunohistochemistry with anti-Npn1 antibodies revealed localization of Npn1 in both WT (a; E16.5, b; E17.5) and PlexinA1 KO (c; E16.5, d; E17.5) brains at E16.5 and E17.5. Scale bars: 200 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31430342), licensed under a CC-BY license. Not internally tested by R&D Systems.