Home / Preso/FRMPD4 / Human/Mouse Preso/FRMPD4 Antibody

Human/Mouse Preso/FRMPD4 Antibody

Catalog #: MAB7676
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7676
MAB7676-SP
Detection of Human and Mouse Preso/FRMPD4 by Western Blot.
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Product Details
Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Preso/FRMPD4 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Preso/FRMPD4 in direct ELISAs and human and mouse Preso/FRMPD4 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 809408
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Preso/FRMPD4
Asn890-Ser1022
Accession # Q14CM0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Simple Western
20 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Preso/FRMPD4 antibody by Western Blot. View Larger

Detection of Human and Mouse Preso/FRMPD4 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Preso/FRMPD4 Monoclonal Antibody (Catalog # MAB7676) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Preso/FRMPD4 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Mouse Preso/FRMPD4 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Preso/FRMPD4 by Simple WesternTM. Simple Western lane view shows lysates of bEnd.3 mouse endothelioma cell line, loaded at 0.5 mg/mL. A specific band was detected for Preso/FRMPD4 at approximately 170 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse Preso/FRMPD4 Monoclonal Antibody (Catalog # MAB7676). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Preso/FRMPD4

Preso, also known as FRMPD4 and PDZD10, is a scaffolding protein that is widely expressed in the brain as 140 kDa and 170 kDa species. It contains one WW domain (aa 33-66) one PDZ domain (aa 78-155), and one FERM domain (aa 204-519). The 140 kDa species is enriched in post-synaptic densities where it interacts with PSD-95, SAP97, SAP102, and F-actin. Preso promotes dendritic spine extension and maintenance as well as excitatory synaptic transmission. Within aa 890-1022, human Preso shares approximately 94% aa sequence identity with mouse and rat Preso.

Long Name
FERM and PDZ Domain Containing 4
Entrez Gene IDs
9758 (Human); 333605 (Mouse); 302656 (Rat)
Alternate Names
FERM and PDZ domain containing 4; FERM and PDZ domain-containing protein 4; FRMPD4; MGC142260; MGC142262; PDZ domain containing 10; PDZD10; PDZK10; Preso

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Citation for Human/Mouse Preso/FRMPD4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Preso regulates NMDA receptor-mediated excitotoxicity via modulating nitric oxide and calcium responses after traumatic brain injury
    Authors: P Luo, X Li, X Wu, S Dai, Y Yang, H Xu, D Jing, W Rao, H Xu, X Gao, Z Fei, H Lu
    Cell Death Dis, 2019-06-24;10(7):496.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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