Human/Mouse/Primate Dopamine beta-Hydroxylase C-Terminus Antibody

Catalog #: PPS066 Datasheet / COA / SDS

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PPS066

Detection of Dopamine beta -Hydroxylase (C-Terminus) by Western blot.

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Human/Mouse/Primate Dopamine beta-Hydroxylase C-Terminus Antibody Summary

Species Reactivity

Human, Mouse, Primate

Specificity

Human, monkey, bovine, and rabbit ~75 kDa Dopamine beta -hydroxylase (D beta H)

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Dopamine beta-Hydroxylase

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, and 0.09% sodium azide.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Dopamine beta -Hydroxylase (C-Terminus) by Western blot. Western blot of human adrenal medulla lysate showing specific immunolabeling of the ~75 kDa D beta H protein.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: Dopamine beta-Hydroxylase

Dopamine beta -hydroxylase (D beta H; also dopamine beta -monooxygenase) is a 73-77 kDa member of the copper type II ascorbate-dependent monooxygenase family. It is both soluble (73 kDa) and membrane-bound (77 kDa) (anchored by an uncleaved signal sequence), and via hydroxylation, converts dopamine into norepinepherine. Human D beta H is a copper-containing disulfide-linked homodimer that is found in neurons and adrenal medullary cells. It is 603 amino acids (aa) in length and contains a 25 aa signal sequence followed by three domains. The first is an N-terminal 120 aa DOMON domain (dopamine beta -monooxygenase N-terminal) that may either bind D beta H to the cell membrane, or participate in tetramerization. This is followed by two 150 aa Cu⁺-type II ascorbate-dependent monooxygenase domains (aa 182-330 and 352-512). D beta H may be most active as a dimeric-dimer/tetramer, whose association status is dependent on local Cl^- concentrations.

References

Kreek, M.J. et al. (2005) Pharmacol. Rev. 57:1.
Stewart, L. and J.P. Klinman (1999) FEBS Lett. 454:229.
Houhou, L. et al. (1995) J. Biol. Chem. 270:12601.
Timmers, H. et al. (2004) Ann. N.Y. Acad. Sci. 1018:520.
Lamouroux, A. et al. (1987) EMBO J. 6:3931.

Entrez Gene IDs

1621 (Human); 13166 (Mouse); 25699 (Rat)

Alternate Names

DBH; DBM; dopamine beta-hydroxylase (dopamine beta-monooxygenase); Dopamine betaHydroxylase; Dopamine beta-Hydroxylase; Dopamine beta-monooxygenase; DOPBHY; EC 1.14.17.1

Product Datasheets

Product Datasheet

COA

SDS

Product Specific Notices

* This product contains sodium azide, which may react with lead and copper plumbing to form explosive metallic azides. Flush with large volumes of water during disposal.