Human/Mouse/Rat alpha-Internexin Antibody

Catalog #: AF4844 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4844
AF4844-SP

Detection of Human/Mouse/Rat alpha ‑Internexin by Western Blot.

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Human/Mouse/Rat alpha-Internexin Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse, and rat alpha -Internexin in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human alpha -Internexin
Val230-Glu450
Accession # Q16352

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat alpha ‑Internexin by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, human, mouse, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat a-Internexin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4844) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha-Internexin at approximately 66 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Internexin

Alpha-Internexin ( alpha -INX; also 66 kDa neurofilament protein (NF-66)) is a 66 kDa member of the intermediate filament (IF) protein family. It was the first of two molecules named for its presumed interaction with cytoskeletal proteins. alpha -INX is one of four Class IV neuronal IF proteins. It both self-assembles, and complexes with NF-L, H and M in select cortical and cerebellar neurons. Human alpha -INX is 499 amino acids (aa) in length. It has a predicted MW of 55 kDa, but runs anomalously at 66 kDa in SDS-PAGE. alpha -INX contains one DNA-binding region (aa 10‑92), a poly-Glu segment (aa 449‑454) and three serine phosphorylation sites (Ser72/335/496). There is one 494 aa alternative splice form that shows multiple short sequence aa substitutions in the first 200 amino acids. Over aa 230‑450, human alpha -INX shares more that 96% aa identity with mouse, canine and rat alpha -INX.

Entrez Gene IDs

9118 (Human); 226180 (Mouse); 24503 (Rat)

Alternate Names

66 kDa neurofilament protein; alphaInternexin; alpha-Internexin; alpha-Inx; FLJ18662; FLJ57501; INA; internexin neuronal intermediate filament protein, alpha; MGC12702; NEF5; neurofilament 5 (66kD); Neurofilament 5; Neurofilament-66; neurofilament-66, tax-binding protein; NF-66; NF-66alpha-internexin; TXBP-1