Human/Mouse/Rat alpha Tubulin Antibody Summary
Accession # Q71U36
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat alpha Tubulin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human/Mouse/Rat a Tubulin Monoclonal Antibody (Catalog # MAB9344) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a Tubulin at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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alpha Tubulin in SH‑SY5Y Human Cell Line. a Tubulin was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human/Mouse/Rat a Tubulin Monoclonal Antibody (Catalog # MAB9344) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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alpha Tubulin in Human Brain. a Tubulin was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human/Mouse/Rat a Tubulin Monoclonal Antibody (Catalog # MAB9344) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha Tubulin
Tubulin alpha-1A chain (TUBA1A), also known as Alpha-tubulin 3, is the principal alpha tubulin in morphologically differentiated neurons. Alpha tubulin dimerizes with beta Tubulin to form microtubules. Microtubules mediate transport of proteins and endosomes within cells, and TUBA1A has been shown to interact with metabotropic glutamate receptor 7 (receptor trafficking), synuclein alpha (synaptic plasticity), and N-syndecan (neurite outgrowth). This gene is mutated in malformations of cortical development, including Lissencephaly resulting in microcephaly, developmental delay and early-onset epileptic seizures.
Product Datasheets
Citations for Human/Mouse/Rat alpha Tubulin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Expression of glucocorticoid receptor and HDACs in airway smooth muscle cells is associated with response to steroids in COPD
Authors: Zhou, L;Roth, M;Papakonstantinou, E;Tamm, M;Stolz, D;
Respiratory research
Species: Human
Sample Types: Cell Lysates
Applications: Westen Blot -
Extracellular Heat Shock Protein 70 Increases the Glucocorticoid Receptor and Dual-Specificity Phosphatase 1 via Toll-like Receptor 4 and Attenuates Inflammation in Airway Epithelial Cells
Authors: Zhou, L;Fang, L;Tamm, M;Stolz, D;Roth, M;
International journal of molecular sciences
Species: Human
Sample Types: Cell Lystates
Applications: Western Blot -
CRIF1 siRNA-Encapsulated PLGA Nanoparticles Suppress Tumor Growth in MCF-7 Human Breast Cancer Cells
Authors: S Piao, I Lee, S Kim, H Park, H Nagar, SJ Choi, GH Vu, M Kim, EO Lee, BH Jeon, DW Kim, Y Seo, CS Kim
International Journal of Molecular Sciences, 2023-04-18;24(8):.
Species: Human, Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Suppressing gain-of-function proteins via CRISPR/Cas9 system in SCA1 cells
Authors: M Pappadà, O Bonuccelli, M Buratto, R Fontana, M Sicurella, A Caproni, S Fuselli, A Benazzo, R Bertorelli, V De Sanctis, P Cavallerio, V Simioni, V Tugnoli, F Salvatori, P Marconi
Scientific Reports, 2022-11-24;12(1):20285.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Characterization of SARS-CoV-2 Spike mutations important for infection of mice and escape from human immune sera
Authors: R Rathnasing, S Jangra, C Ye, A Cupic, G Singh, C Martínez-R, LCF Mulder, T Kehrer, S Yildiz, A Choi, ST Yeung, I Mena, V Gillespie, J De Vrieze, S Aslam, D Stadlbauer, DA Meekins, CD McDowell, V Balaraman, MJ Corley, JA Richt, BG De Geest, L Miorin, PVI study, F Krammer, L Martinez-S, V Simon, A García-Sas, M Schotsaert
Nature Communications, 2022-07-07;13(1):3921.
Species: African Green Monkey
Sample Types: Cell Lysates
Applications: Western Blot -
SIRT1 Activation Attenuates the Cardiac Dysfunction Induced by Endothelial Cell-Specific Deletion of CRIF1
Authors: S Piao, I Lee, SA Jin, S Kim, H Nagar, SJ Choi, BH Jeon, CS Kim
Biomedicines, 2021-01-08;9(1):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Prolactin Acts on Myeloid Progenitors to Modulate SMAD7 Expression and Enhance Hematopoietic Stem Cell Differentiation into the NK Cell Lineage
Authors: DM Tufa, T Shank, AM Yingst, GD Trahan, S Shim, J Lake, R Woods, K Jones, MR Verneris
Sci Rep, 2020-04-14;10(1):6335.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Collaboration Between RSK-EphA2 and Gas6-Axl RTK Signaling in Arginine Starvation Response That Confers Resistance to EGFR Inhibitors
Authors: MT Kuo, Y Long, WB Tsai, YY Li, HHW Chen, LG Feun, N Savaraj
Transl Oncol, 2019-12-27;13(2):355-364.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Targeting Toll-like receptors with soluble Toll-like receptor 2 prevents peritoneal dialysis solution-induced fibrosis.
Authors: Raby A, Gonzalez-Mateo G, Williams A, Topley N, Fraser D, Lopez-Cabrera M, Labeta M
Kidney Int, 2018-05-31;94(2):346-362.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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Paraformaldehyde-fixed fibroblast stained with alpha tubulin (red), and counterstained with DAPI
different protein quantities of an MSC lysate were used to test the antibody.
