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Human/Mouse/Rat AMPK alpha 1 Antibody

Catalog #: AF3197
13 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3197
AF3197-SP
AMPK alpha 1 in Human Liver.
3 Images
Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat AMPK alpha 1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat AMPK alpha 1 in Western blots. The antibody does not cross-react with recombinant human AMPK alpha 2.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human AMPK alpha 1
Lys349-Gln559
Accession # Q13131
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry AMPKa1 antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

AMPK alpha 1 in Human Liver. AMPKa1 was detected in immersion fixed paraffin-embedded sections of human liver using 5 µg/mL Goat Anti-Human/Mouse/Rat AMPKa1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3197) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows secondary antibody only control experiment. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human. Mouse, and Rat AMPKa1 antibody by Western Blot. View Larger

Detection of Human. Mouse, and Rat AMPK alpha 1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse/Rat AMPKa1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for AMPKa1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry AMPKa1 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

AMPK alpha 1 in MCF‑7 Human Cell Line. AMPKa1 was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human/Mouse/Rat AMPKa1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3197) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMPK alpha 1

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic  alpha subunit and regulatory beta  and gamma  subunits. Each subunit exists as alternate isoforms ( alpha 1,  alpha 2, beta 1,  beta 2,  gamma 1,  gamma 2,  gamma 3), with all 12 combinations able to form complexes. The catalytic alpha  subunit of AMPK is activated allosterically by AMP, and by phosphorylation via the AMPK kinases LKB1 and CaMKK beta. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.

Long Name
AMP-activated Protein Kinase alpha 1
Entrez Gene IDs
5562 (Human)
Alternate Names
AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK alpha 1; AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain; AMPK subunit alpha-1; AMPK; AMPK1; AMPKa1; EC 2.7.11; EC 2.7.11.1; MGC33776; MGC57364; PRKAA1; protein kinase, AMP-activated, alpha 1 catalytic subunit

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Citations for Human/Mouse/Rat AMPK alpha 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Metformin Promotes Antitumor Immunity via Endoplasmic-Reticulum-Associated Degradation of PD-L1
    Authors: Cha JH, Yang WH, Xia W et al.
    Mol. Cell
  2. Tetraspanin 1 promotes endometriosis leading to ovarian clear cell carcinoma
    Authors: Ha‐Yeon Shin, Wookyeom Yang, Doo Byung Chay, Eun‐ju Lee, Joon‐Yong Chung, Hyun‐Soo Kim et al.
    Molecular Oncology
  3. Empagliflozin Ameliorates Preeclampsia and Reduces Postpartum Susceptibility to Adriamycin in a Mouse Model Induced by Angiotensin Receptor Agonistic Autoantibodies
    Authors: Ruonan Zhai, Yuan Liu, Jiahao Tong, Ying Yu, Lin Yang, Yong Gu et al.
    Frontiers in Pharmacology
  4. Brain-specific repression of AMPKalpha1 alleviates pathophysiology in Alzheimer's model mice
    Authors: HR Zimmermann, W Yang, NP Kasica, X Zhou, X Wang, BC Beckelman, J Lee, CM Furdui, CD Keene, T Ma
    J. Clin. Invest., 2020-07-01;130(7):3511-3527.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. TOM40 Inhibits Ovarian Cancer Cell Growth by Modulating Mitochondrial Function Including Intracellular ATP and ROS Levels
    Authors: W Yang, HY Shin, H Cho, JY Chung, EJ Lee, JH Kim, ES Kang
    Cancers (Basel), 2020-05-22;12(5):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Brain-specific suppression of AMPKalpha2 isoform impairs cognition and hippocampal LTP by PERK-mediated eIF2alpha phosphorylation
    Authors: W Yang, X Zhou, HR Zimmermann, T Ma
    Mol. Psychiatry, 2020-05-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Disrupted structure and aberrant function of CHIP mediates the loss of motor and cognitive function in preclinical models of SCAR16
    Authors: CH Shi, C Rubel, SE Soss, R Sanchez-Ho, S Zhang, SC Madrigal, S Ravi, H McDonough, RC Page, WJ Chazin, C Patterson, CY Mao, MS Willis, HY Luo, YS Li, DA Stevens, MB Tang, P Du, YH Wang, ZW Hu, YM Xu, JC Schisler
    PLoS Genet., 2018-09-17;14(9):e1007664.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  8. Modulating the therapeutic response of tumours to dietary serine and glycine starvation
    Authors: ODK Maddocks, D Athineos, EC Cheung, P Lee, T Zhang, NJF van den Br, GM Mackay, CF Labuschagn, D Gay, F Kruiswijk, J Blagih, DF Vincent, KJ Campbell, F Ceteci, OJ Sansom, K Blyth, KH Vousden
    Nature, 2017-04-19;544(7650):372-376.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. AMP-activated protein kinase modulates tau phosphorylation and tau pathology in vivo
    Authors: Manon Domise
    Sci Rep, 2016-05-27;6(0):26758.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  10. Mitochondria and AMP-activated protein kinase-dependent mechanism of efferocytosis.
    Authors: Jiang S, Park D, Stigler W, Creighton J, Ravi S, Darley-Usmar V, Zmijewski J
    J Biol Chem, 2013-07-29;288(36):26013-26.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Regulation of AMP-activated protein kinase signaling by AFF4 protein, member of AF4 (ALL1-fused gene from chromosome 4) family of transcription factors, in hypothalamic neurons.
    Authors: Komori T, Doi A, Nosaka T, Furuta H, Akamizu T, Kitamura T, Senba E, Morikawa Y
    J. Biol. Chem., 2012-04-23;287(24):19985-96.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. Adenosine Monophosphate-activated Protein Kinase (AMPK) in serotonin neurons mediates select behaviors during protracted withdrawal from morphine in mice
    Authors: Darrell Eacret, Julia Noreck, Julie A. Blendy
    Behavioural Brain Research
  13. Melatonin: The smart molecule that differentially modulates autophagy in tumor and normal placental cells
    Authors: Lucas Sagrillo-Fagundes, Josianne Bienvenue-Pariseault, Cathy Vaillancourt
    PLOS ONE

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Human/Mouse/Rat AMPK alpha 1 Antibody
By Anonymous on 10/17/2018
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human
Western Blot Human/Mouse/Rat AMPK alpha 1 Antibody AF3197