Human/Mouse/Rat Annexin A11 Antibody

Catalog #: AF3927 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3927
AF3927-SP

Detection of Human/Mouse/Rat Annexin A11 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Annexin A11 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse, and rat Annexin A11 in Western blots. In Western blots, this antibody shows no cross-reactivity with recombinant human Annexin A1, A2, A3, A4, A5, A6, A7, A8, A9, A10, or A13.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Annexin A11
Met1-Asp505
Accession # P50995

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat Annexin A11 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Annexin A11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Annexin A11 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western

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Detection of Human Annexin A11 by Simple Western^TM. Simple Western shows lysates of Exosome Standards (LNCaP) (NBP3-11687), Exosome Standards (PC‑3) (NBP2-49856) and A431 human epithelial carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Annexin A11 at approximately 59 kDa (as indicated) using 50 µg/mL of Goat Anti-Human/Mouse/Rat Annexin A11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3927). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Annexin A11

Annexin A11 (ANXA11), also known as Annexin XI, 56K Autoantigen and Calcyclin-associated annexin 50 (CAP50), is a 54.4 kDa (predicted) member of the Annexin protein family. The Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexin’s have a molecular weight of approximately 35‑40 kDa and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites. The C‑terminal domain is comprised of four 60‑70 amino acid (aa) repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats). The four annexin repeats form a highly alpha -helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner. Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. Annexin A11 was identified as a 56 kDa antigen recognized by sera from patients with autoimmune diseases such as rheumatoid arthritis, systemic lupus erythematosus, or Sjogren's syndrome. Human Annexin A11 shares 93% identity with mouse and rat Annexin A11.

Entrez Gene IDs

311 (Human); 11744 (Mouse); 290527 (Rat)

Alternate Names

Annexin A11; Annexin-11; ANX1156-kD; ANXA11; CAP50; CAP-50